| 羊口疮病毒疫苗株和野毒株F1L基因的比较分析 |
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| 引用本文： | 王文莉,魏楠楠,徐守兴,卢炳洲,张大俊,张克山,郑海学,刘湘涛. 羊口疮病毒疫苗株和野毒株F1L基因的比较分析[J]. 中国动物传染病学报, 2019, 0(4): 83-87 |
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| 作者姓名： | 王文莉 魏楠楠 徐守兴 卢炳洲 张大俊 张克山 郑海学 刘湘涛 |
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| 作者单位： | 甘肃省动物疫病预防控制中心;中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室农业部畜禽病毒学重点开放实验室国家口蹄疫参考实验室 |
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| 基金项目： | 国家重点研发计划(2018YFD0502100);国家自然科学基金(31201914) |
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| 摘 要： | 羊口疮(Orf)是由羊口疮病毒(Orf virus,ORFV)引起的人兽共患传染病,ORFV F1L基因编码肝素结合蛋白。为比较分析ORFV疫苗株和野毒株编码的F1L基因的特征,本试验对疫苗株和野毒株的F1L基因进行PCR扩增、克隆及序列测定,并应用生物信息学相关软件及方法,对两者的F1L基因在核酸水平和氨基酸水平的变异情况及其二、三级蛋白结构变化情况进行了比较分析。结果显示,此次测定的ORFV疫苗株与野毒株F1L基因核苷酸序列相似性为95.9%,氨基酸序列相似性为94.5%。本研究结果为揭示ORFV异源宿主致弱的机制积累了资料。
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| 关 键 词： | 羊口疮病毒 疫苗株 野毒株 F1L基因 |
COMPARATIVE ANALYSIS OF F1L GENES IN VACCINE AND FIELD STRAINS OF ORF VIRUS |
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| WANG Wen-li,WEI Nan-nan,XU Shou-xing,LU Bing-zhou,ZHANG Da-jun,ZHANG Ke-shan,ZHENG Hai-xue,LIU Xiang-tao. COMPARATIVE ANALYSIS OF F1L GENES IN VACCINE AND FIELD STRAINS OF ORF VIRUS[J]. Chinese Journal of Animal Infectious Diseases, 2019, 0(4): 83-87 |
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| Authors: | WANG Wen-li WEI Nan-nan XU Shou-xing LU Bing-zhou ZHANG Da-jun ZHANG Ke-shan ZHENG Hai-xue LIU Xiang-tao |
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| Affiliation: | (Gansu Animal disease control center,Lanzhou 730070,China;Ministry of Agriculture National Foot and Mouth Disease Reference Laboratory,Key Laboratory of Animal Virology,State Key Laboratory of Veterinary Etiological Biology,Lanzhou Veterinary Research Institute,CAAS,Lanzhou 730046,China) |
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| Abstract: | Orf is an infectious zoonosis caused by orf virus (ORFV).F1L gene of ORFV encodes a heparin-binding protein.To identify the difference between ORFV vaccine and wild strain,F1L gene was amplified by PCR.The nucleotide and amino acid sequences as well as their secondary and three-dimensional structures were analyzed using bioinformatics software.The results showed that the nucleotide sequence similarity of F1L gene of ORFV vaccine strain was 95.9% to wild strain and amino acid sequence was 94.5%.The results from this study provided useful information for further investigation into ORFV attenuation mechanism by passaging in heterogeneous hosts. |
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| Keywords: | Orf virus vaccine strain wild strain F1L gene |
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