EMS诱变甘蓝型油菜获得高油酸突变体

用0.4％ EMS诱变甘蓝型油菜NJ7982种子,从M2代筛选得到1个油酸含量76.15％的突变株(M2-2080),M3代该突变体的油酸含量也达到了75.0％ (M3-2080-1).对高油酸突变体M3-2080-1和野生型BnFA D2基因进行克隆,分析突变体油酸含量高的原因.序列表明,高油酸突变体中,BnFA D...

High Oleate Mutants of Brassica napus Produced by EMS Inducement

Seeds of NJ7982 (Brassica napus L.) were treated with 0.4% EMS, and one high oleate plant (76.15%) was obtained through screening in M2 generation. In its M3 progenies, the highest oleate content reached 75.02% (M3-2080-1). The BnFAD2 genes of the high oleate mutant M3-2080-1 and the wild type were cloned for revealing the causes of seed high oleate content. Result showed that 63 nucleotides in BnFAD2I gene were mutated, resulting in translation termination of its functional protein; 41 bases in the BnFAD2II-1 gene had been mutated, leading to the change of 15 amino acids, of which include residue Seu-82 and Phe-157 locating in transmembrane region and hydrophobic area respectively were substituted into a Proline and a Leucine, respectively, and 9 amino acids had mutations in the α-helix area. The mutations taking place in the conserved regions considered to be the catalytic center of enzyme, are thought to possibly inactivate the desaturase. There is no significant difference between the high oleate material and the wild type agronomic character means, therefor, it could be used as high oleate germplasm for breeding.