基于RPA技术对转CP4-EPSPS基因产品的快速检测

核酸检测在农产品安全检测中的应用十分广泛，随着转基因作物的商业化种植，迫切需要一种快速、特异、灵敏的转基因作物检测方法。利用重组酶聚合酶扩增（recombinase polymerase amplification，RPA）技术对含有转基因产品及其制品中CP4-EPSPS基因进行检测，共设计5对引物来筛取最佳引物，并对反应体系和反应温度进行优化。结果表明，该方法采用20 μL体系在37 ℃恒温反应15 min即可对CP4-EPSPS基因进行快速检测。该方法检测转基因的灵敏度阈值为45拷贝，灵敏度高、特异性强，为大规模筛查CP4-EPSPS基因提供了一种新的途径。

Rapid Detection of CP4-EPSPS Transgenic Products Based on RPA Technology

Nucleic acid detection is widely used in the safety detection of agricultural products. With the commercial cultivation of genetically modified crops， the method with rapid， specific and sensitive is an urgent need for the detection of genetically modified crops. The recombinase polymerase amplification （RPA） technology was used to detect CP4-EPSPS genes in genetically modified products and their products. Five pairs of primers were designed to screen the best pair of primers， and the reaction system and temperature were optimized. The results show that CP4-EPSPS gene could be quickly detected by this method with 20 μL reaction system， 15 min reacted at 37 ℃. The sensitivity threshold for detecting transgenic DNA was 45 copies with highly sensitive and specific nucleic， which provided a new way for large-scale screening of CP4-EPSPS gene.