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First report of Helleborus net necrosis virus isolated from hellebores with black death syndrome in Japan |
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| Authors: | Takuya Shiraishi Hideo Hoshi Koki Eimori Takeshi Kawanishi Ken Komatsu Masayoshi Hashimoto Kensaku Maejima Yasuyuki Yamaji Shigetou Namba |
| Institution: | (1) Department of Agricultural and Environmental Biology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan;(2) Tokyo Metropolitan Agriculture and Forestry Research Center, 3-8-1 Fujimi-cho, Tachikawa, Tokyo 190-0013, Japan; |
| Abstract: | Helleborus net necrosis virus (HeNNV) in hellebores (Helleborus spp.) has been detected for the first time in Japan. Infected plants had black streaks on fresh leaves and black spots on sepals, which resembled the symptoms of black death disease. The morphology of the virus particles isolated from infected plants was comparable to that of carlaviruses. RT-PCR analysis showed that the entire HeNNV genomic sequence isolated in Japan shared 99% identity with that of HeNNV previously reported in the United States. |
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