小型西瓜SRAP技术体系优化

为探讨小型西瓜种质遗传分析奠定基础以及不类型西瓜SRAP技术体系的通用性，以小型西瓜F1‘秀丽’为试材，利用正交试验设计，对SRAP-PCR反应体系中的Mg2+浓度、dNTPs浓度、引物浓度、Taq聚合酶浓度和模板DNA浓度进行5因素4水平的筛选分析，用Me3-Em3引物组合进行PCR扩增以确定最优反应体系；进一步应用该优化反应体系，对5个不同引物和37份不同果型西瓜资源DNA进行SRAP-PCR扩增。结果表明，小型西瓜SRAP-PCR最佳反应体系为：10× PCR buffer 2 μL、Mg2+ 3.0 mmol/L，dNTPs 0.2 mmol/L，引物0.5 μmol/L，模板DNA 40 ng、Taq聚合酶0.5 U，总体积为10 μL。不同果型西瓜资源DNA进行SRAP-PCR扩增，电泳条带清晰、稳定性好，说明不同果型西瓜种质SPAP体系具有通用性。

Optimization of SRAP Reaction System on Mini-watermelon

To establish SRAP reaction system for mini-watermelon and explore the generality of the SRAP technology system among different type of watermelon resources,the SRAP reaction system was optimized,in terms of Mg 2+,dNTPs,primers,Taq polymerase and template DNA 5 factors and their 4 levels with the primer Em3-Me3,by an orthogonal design.And further application of the optimal reaction system was applied among different type of watermelon resources.The results indicated that the watermelon appropriate SRAP-PCR r...