| 口蹄疫病毒RNA复制酶基因3D的克隆、表达及纯化 |
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| 引用本文： | 孙涛,陆苹.口蹄疫病毒RNA复制酶基因3D的克隆、表达及纯化[J].中国兽医学报,2005,25(2):119-121. |
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| 作者姓名： | 孙涛 陆苹 |
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| 作者单位： | 上海交通大学,农业生物技术学院,生物技术研究所,上海,201101 |
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| 摘 要： | 利用RT-PCR扩增了口蹄疫病毒(FMDV)的RNA复制酶基因，并将其克隆到原核表达载体pET-32a( )中。重组质粒在大肠杆菌中表达后的目的蛋白为可溶性形式，纯化产物用感染A型FMDV的豚鼠康复血清进行Western-blotting检测，结果表明，3D基因得到了正确的表达。
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| 关 键 词： | 口蹄疫病毒 RNA复制酶基因 基因表达 基因克隆 基因纯化 |
| 文章编号： | 1005-4545(2005)02-0119-03 |
| 修稿时间： | 2003年11月3日 |
Cloning,Expression and Purification of Replicase Gene of Foot-and-Mouth Disease Virus |
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| SUN Tao,LU Ping.Cloning,Expression and Purification of Replicase Gene of Foot-and-Mouth Disease Virus[J].Chinese Journal of Veterinary Science,2005,25(2):119-121. |
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| Authors: | SUN Tao LU Ping |
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| Abstract: | The RNA replicase gene of foot-and-mouth virus was amplified and cloned into prokaryotic expression vector pET-32a(+).The recombinant plasmid was transformed into E.coli and the target protein was expressed in soluble form.The purified recombinant protein was substantiated to be expressed correctly by Western-blotting. |
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| Keywords: | foot-and-mouth virus RNA replicase gene expression |
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