| 山东桔梗多倍体诱导与鉴定 |
| |
| 引用本文： | 朱京斌,单成钢,王维婷,王志芬.山东桔梗多倍体诱导与鉴定[J].山东农业科学,2013(10):32-34. |
| |
| 作者姓名： | 朱京斌 单成钢 王维婷 王志芬 |
| |
| 作者单位： | 山东省农业科学院农产品研究所／药用植物研究中心,山东济南250100 |
| |
| 基金项目： | 山东省农业良种工程重大课题（2011LZ001-3）资助. |
| |
| 摘 要： | 在离体培养条件下，比较了单用不同浓度秋水仙素和0．2％秋水仙素与不同浓度二甲基亚砜（DMSO）混用对山东桔梗愈伤组织诱导多倍体的效果。结果表明：用0．2％秋水仙素和2％DMSO浸泡山东桔梗愈伤组织16h，多倍体诱导率最高，达43．3％。桔梗多倍体植株叶颜色深、有皱褶，叶片大且厚，茎秆粗壮，节间距长。经显微鉴定，多倍体叶气孔增大，但单位面积气孔数目减少。
|
| 关 键 词： | 桔梗 秋水仙素 二甲基亚砜 多倍体诱导 |
Polyploid Induction and Identification of Shandong Platycodon grandiflorum |
| |
| Zhu Jingbin,Shan Chenggang,Wang Weiting,Wang Zhifen.Polyploid Induction and Identification of Shandong Platycodon grandiflorum[J].Shandong Agricultural Sciences,2013(10):32-34. |
| |
| Authors: | Zhu Jingbin Shan Chenggang Wang Weiting Wang Zhifen |
| |
| Institution: | (Institute of Agro -Food Science and Technology/Research Center of Medicinal Plants, Shandong Academy of Agricultural Sciences ,Jinan 250100, China) |
| |
| Abstract: | The polyploid induction effects of only using different concentrations of cochicine, and mixedly using 0.2% cochicine with different concentrations of dimethyl sulfoxide（DMSO） on Shandong Platycodon grandiflorum callus were studied in vitro. The results showed that the callus immersed in 0.2% colchicine and 2% DMSO for i6 hours had the highest polyploid induction rate as 43.3%. The polyploid plants had thick, large and dark leaves with wrinkles, strong stems and long internode length. By microscopic identification, the polyploid leaves had larger stomatal size but fewer stomatal number per unit area. |
| |
| Keywords: | Platycodon grandiflorum Cochieine Dimethyl sulfoxide Polyploid induction |
| 本文献已被 维普 等数据库收录！ |
