| 茶树花色素还原酶基因的克隆与原核表达 |
| |
| 引用本文： | 郑华坤,黄志伟,颜霜飞,郑金贵. 茶树花色素还原酶基因的克隆与原核表达[J]. 福建农林大学学报(自然科学版), 2008, 37(6) |
| |
| 作者姓名： | 郑华坤 黄志伟 颜霜飞 郑金贵 |
| |
| 作者单位： | 福建农林大学农产品品质研究所,福建,福州,350002 |
| |
| 基金项目： | 国家重大基础研究前期研究专项项目,福建省科技厅重大专项前期研究项目,福建省科技创新平台建设项目 |
| |
| 摘 要： | 采用RACE技术,从茶树新品系"1005"的嫩芽中克隆出ANR基因的全长cDNA(1260 bp),其推导的氨基酸序列与茶树、葡萄花色素还原酶的一致性分别为99%和84%;并成功构建了ANR基因的原核表达载体pET-ANR,在大肠杆菌BL21中表达出预期大小(约45 ku)的融合蛋白.
|
| 关 键 词： | 茶树 花色素还原酶 基因克隆 原核表达 |
Cloning and prokaryotic expression of anthocyanidin reductase gene from Camellia sinensis |
| |
| ZHENG Hua-kun,HUANG Zhi-wei,YAN Shuang-fei,ZHENG Jin-gui. Cloning and prokaryotic expression of anthocyanidin reductase gene from Camellia sinensis[J]. Journal of Fujian Agricultural and Forestry University, 2008, 37(6) |
| |
| Authors: | ZHENG Hua-kun HUANG Zhi-wei YAN Shuang-fei ZHENG Jin-gui |
| |
| Abstract: | The full-length cDNA(1260 bp) of anthocyanidin reductase gene was cloned from Camellia sinensis "1005" using RACE technique.Its putative amino acid sequence displays 99% and 84% similarity to that of C.sinensis and Viti vinifera ANR.Then,the recombinant pET-ANR was constructed to express anthocyanidin reductase gene in E.coli BL21.The fusion protein with an expected weight(45 ku) was detected by SDS-PAGE. |
| |
| Keywords: | Camellia sinensis anthocyanidin reductase gene cloning prokaryotic expression |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
