| 鸡毒霉形体S6株的致弱研究 |
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| 引用本文： | 邵国青,蔡宝祥.鸡毒霉形体S6株的致弱研究[J].南京农业大学学报,1997,20(2):90-94. |
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| 作者姓名： | 邵国青 蔡宝祥 |
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| 作者单位： | [1]江苏省农科院畜牧兽医研究所 [2]南京农业大学动物医学院 |
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| 摘 要： | 鸡毒霉形体（ＭＧ）中毒株Ｓ６在固体平板上的３次克隆后，在无细胞培养上基上传１００代以上进行致弱培养，用ＭＧＳ６Ｆ１０８株培养物在雏物和ＳＰＦ鸡胚上作６次回归感染，证明ＭＧＳ６Ｆ１０８不返强，通过气管环培养，纤毛运动损伤试验及对鸡点眼，滴鼻，工胸气囊注射不同代培养物（Ｆ１０８，Ｆ１４３，Ｆ１２１，Ｆ１５０，Ｆ１５３）检查气囊病变和病原体再分离，结果证明Ｆ１０８以上代次的菌株低毒，安全，且有较强的免疫
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| 关 键 词： | 鸡毒霉形体 菌株 气管环培养 克隆 致弱 |
ATTENUATION OF MYCOPLASMA GALLISEPTICUM S 6 STRAIN |
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| Shao Guoqing,Mao Hongxian,Cai Baoxiang,Qian Jianfei and Lu Lixin.ATTENUATION OF MYCOPLASMA GALLISEPTICUM S 6 STRAIN[J].Journal of Nanjing Agricultural University,1997,20(2):90-94. |
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| Authors: | Shao Guoqing Mao Hongxian Cai Baoxiang Qian Jianfei and Lu Lixin |
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| Abstract: | After 3 sequential cloning in PPLO agar plate, laboratory attenuated strain F 108 of M. gallisepticum , derived from strain S 6 was selected. By more than 100 in vitro passages, cloned strain F 108 did not revert to the former level of pathogenicity in 6 back passages in chickens. With the observations of pathogenic effects on chick tracheal ring culture, and with aerosol challenge and ocular instillation, F 108 F 113 F 121 F 150 F 153 could induce significant serological response and were weakly virulent. |
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| Keywords: | Mycoplasma gallisepticum strain tracheal ring culture back passage clone attenuation |
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