微卫星标记与商品代固始鸡体型参数和屠体性状的关联性分析

利用最小二乘法拟合线性模型,探索了与鸡体型参数和屠体性状紧密相关的10个微卫星座位与商品代固始鸡体型参数和屠体性状的关系。用变性聚丙烯酰胺凝胶电泳法,分析了这10个微卫星位点在100只商品代固始鸡中的遗传变异,计算了微卫星位点的等位基因频率、杂合度、多态信息含量和有效等位基因数。结果表明,其中8对微卫星引物表现出多态,平均每个座位检测到3.625个等位基因(3~4个),平均多态信息含量为0.520 3,平均杂合度为0.626 0。通过最小二乘分析,检测到MCW0295、MCW0006、MCW0185、ADL0192位点与体型参数中的5种性状间存在不同程度的关联性;MCW0006、MCW0185、ADL0105位点与商品代固始鸡6个屠体性状中的4个性状显著连锁。MCW0295位点的AC基因型有望作为质量的早期选择辅助标记,MCW0006和MCW0185位点的CC基因型有望作为质量、胸宽、胸深、胫长、体斜长的早期选择辅助标记,ADL0192位点的AB基因型有望作为质量、胸宽的早期选择辅助标记;MCW0006位点的CC基因型有望作为全净膛率的选择辅助标记,MCW0185位点的AD基因型有望作为胸肌率和腿肌率的选择辅助标记,ADL0105位点的CC基因型有望作为腹脂率的选择辅助标记。

Correlation analysis of microsatellite loci for phenotypic traits and slaughter traits in Gushi commercial line

Relationships between microsatellite loci and phenotypic traits and slaughter traits in Gushi commercial line were preliminarily analyzed by least squares linear model. Genetic Variation of ten microsa- tellites which were closely linked to phenotypic traits and slaughter traits was analyzed in 100 Gushi commercial line with denaturing olyacrylamide gelelectrophores. Allele frequency,heterozygosity, polymorphic information content,and the effective numbers of alleles of ten microsatellite loci were calculated. The results indicated eight microsatellite markers were polymorphic with 3. 625 on average. The average PIC was 0. 520 3 and the average heterozigosity was 0. 626 0. The result suggested there were links among MCW0295, MCW0006, MCW0185, ADL0192 microsatellites and 5 phenotypic characters through least square means, and there were linkage among MCW0006, MCW0185, ADL0105 microsatellites and 4 slaughter traits through least square means. The result implied that genetype AC of marker MCW0295 might be regarded as candidate-locus for body weight trait of Gushi commercial line, genetype CC of marker MCW0006 and MCW0185 might be regarded ascandidate-ocus for phenotypic traits of Gushi commercial line, AB of marker ADL0192 might be regarded ascandidate-locus for body weight trait, chest width of Gushi commercial line genetype CC of marker MCW0006 might be regarded as candidate-locus for eviscerated rate of Gushi commercial line;genetype AD of marker MCW0185 might be regarded as candidate-locus for chest muscule percentage and thigh muscule rate,and genetype CC of marker ADI.0105 might be regarded as candidate-locus for abdominal of Gushi commercial line.