| 3株水产动物病原弧菌主要分泌性蛋白的鉴定及其分泌性序列的分析 |
| |
| 引用本文： | 刘笋,王秀华,黄倢.3株水产动物病原弧菌主要分泌性蛋白的鉴定及其分泌性序列的分析[J].水产学报,2011,35(11):1701-1710. |
| |
| 作者姓名： | 刘笋 王秀华 黄倢 |
| |
| 作者单位： | 1. 中国水产科学研究院黄海水产研究所,农业部海洋渔业资源可持续利用重点开放实验室,山东青岛266071;上海海洋大学水产与生命学院,上海201306
2. 中国水产科学研究院黄海水产研究所,农业部海洋渔业资源可持续利用重点开放实验室,山东青岛266071 |
| |
| 基金项目： | 公益性行业科研专项经费项目;国家虾现代产业技术体系(nycytx-46) |
| |
| 摘 要： | 从3株水产动物病原菌鳗弧菌MN、鳗弧菌3101、费氏弧菌培养液中分别获取了分泌性蛋白,进行SDS-PAGE,对表达量较高的5条蛋白区带进行MALDI-TOF/TOF质谱鉴定表明,它们分别是鳗弧菌MN金属蛋白酶(empA-MN)、鳗弧菌3101金属蛋白酶(empA-3101)、锌金属蛋白酶(zincempA)、费氏弧菌VFMJ11_1094蛋白(VFMJ11_1094)和费氏弧菌ES114的外膜蛋白(OMP).根据所鉴定的序列设计引物,分别从鳗弧菌MN、鳗弧菌3101和费氏弧菌中扩增出相应基因,克隆后测定emp-MN、empA-3101、zinc empA、VFMJ11-1094和OMP相应基因的序列,经在线软件SignaIP 3.0分析,确定emp-MN、empA-3101、zinc empA、VFMJ11-1094和OMP均存在不同的分泌性信号肽序列angMN-35、ang3101-35、ang3101-25、vf-38和vf-23,通过在线软件PSORT分析表明,所有信号肽均定位在细胞的周质空间或细胞外膜上.
|
| 关 键 词： | 水产动物病原菌 分泌性蛋白 MALDI-TOF/TOF质谱分析 分泌性序列 细胞定位 |
| 收稿时间： | 3/9/2011 12:00:00 AM |
| 修稿时间： | 2011/6/30 0:00:00 |
Mass spectrometry identification of secreted proteins from 3 aquatic bacterial isolates of animal origin and analysis of their secretive sequences |
| |
| LIU Sun,WANG Xiu hua and HUANG Jie.Mass spectrometry identification of secreted proteins from 3 aquatic bacterial isolates of animal origin and analysis of their secretive sequences[J].Journal of Fisheries of China,2011,35(11):1701-1710. |
| |
| Authors: | LIU Sun WANG Xiu hua and HUANG Jie |
| |
| Institution: | Key Laboratory for Sustainable Utilization of Marine Fisheries Resource, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences,Key Laboratory for Sustainable Utilization of Marine Fisheries Resource, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences |
| |
| Abstract: | The secreted proteins of Vibrio anguillarum MN,V.anguillarum 3101 and Vibrio fischeri which were isolated and conserved by our laboratory,were extracted.The components of secreted proteins were separated by SDS-PAGE.The 5 high expressed protein bands in the SDS-PAGE were identified by mass spectrometry MALDI-TOF/TOF as the metalloprotease of V.anguillarum MN,the metalloprotease of V.anguillarum 3101,the zinc metalloproteinase of V.anguillarum 3101,the hypothetical protein VFMJ11_1094 and outer membrane prot... |
| |
| Keywords: | aquatic bacteria secreted proteins MALDI-TOF/TOF mass spectrometry secretive sequences cell localization |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《水产学报》浏览原始摘要信息 |
| 点击此处可从《水产学报》下载免费的PDF全文 |
|
