三七NAC基因家族全基因组鉴定与表达分析

为明确三七Panax notoginseng NAC转录因子基因家族的分布、功能和结构，通过生物信息学分析法进行鉴定，对其理化特性、染色体位置和进化特征进行分析，并根据RNA-seq数据分析其家族成员的时空表达模式和受黑斑病菌Alternaria panax诱导后的表达情况。结果显示，三七中共有98个NAC基因家族成员，其编码蛋白质长度介于104～882个氨基酸之间，分子量在11.78～100.20 kD之间，等电点在4.12～9.75之间。这98个NAC基因家族不均匀地分布在三七的12条染色体上，其中1号染色体分布最多（16个），而11号染色体上分布最少（1个）。三七NAC基因启动子区域存在与光响应、生长素响应、赤霉素响应及茉莉酸甲酯响应等相关的多种顺式作用元件。NAC基因在三七不同组织及根部不同发育时期均有表达，在受到黑斑病菌侵染的叶片中NAC部分基因家族成员显著上调表达。表明NAC基因家族在三七的生长发育和响应黑斑病菌侵染过程中具有重要作用。

Genome-wide identification and expression pattern of NAC gene family in medicinal plant Panax notoginseng

In order to clarify the distribution, function and structure of medicinal plant Panax notoginseng NAC gene family, the members in the family were identified and the physicochemical characteristics,chromosomal location and evolutionary features of the family were analyzed with bioinformatics method, the temporal and spatial expression patterns of the family members and the induced expression of Alternaria panax were analyzed based on the RNA-seq data. The results showed that there were 98 members of the NAC gene family in P. notoginseng, coding proteins with a length ranging from 104 to 882amino acids, and molecular weight ranging from 11.78 to 100.20 kD and isoelectric point ranging from 4.12 to 9.75. The 98 NAC genes were unevenly distributed on 12 chromosomes. Chr1 has the most genes(16), while chr11 has the fewest genes(1). Multiple cis-acting elements associated with light response, growth hormone response, gibberellin response and methyl jasmonate response are present in the NAC promoter region. NAC genes were expressed in all different tissues and roots of P. notoginseng at different developmental stages, while the expression of NAC in P. notoginseng leaves also showed significantly increased after infection by A. panax. These results suggested that NAC plays an important role in the growth and development of P. notoginseng and in its response to the infection by A. panax.