去势对淮南猪皮下脂肪PPAR信号通路基因表达量的影响

为了研究PPAR信号通路在猪去势诱导的脂肪沉积中的作用,采集去势和非去势淮南猪(共6头,每组各3头)的皮下脂肪组织,提取总RNA后构建文库,利用高通量测序技术检测PPAR通路相关基因表达量,表达差异基因的筛选阈值为P0.05且|log2差异倍数|1。基于mRNA的差异分析以及KEGG富集分析发现,PPAR信号通路中17个基因表达差异达到显著水平,其中14个基因表达量上调、3个基因表达量下调。脑型脂肪酸结合蛋白基因(FABP7)、甘油水孔蛋白基因(AQP7)、视黄素X受体γ基因(RXRγ)等基因上调,而磷酸烯醇丙酮酸羧激酶1基因(PCK1)、过氧化物酶体增殖物激活受体γ辅激活因子1α基因(PPARGC1α)、胆固醇7α-羟化酶1基因(CYP7A1)参与分解代谢的基因显著下调。

Effect of Castration on PPAR Signal Pathway Expression in Huainan Pig Subcutaneous Fat Tissue

To detect the role of PPAR signal pathway on the castration-induced fat deposition,RNA was isola-ted from the subcutaneous fat tissues of three castrated Huainan pigs and three intact Huainan pigs.The high-throughput sequencing approach was used to identify mRNA expression difference in castrated and intact pigs.The screening threshold for these genes was P value <0.05 and |log2 FoldChange |>1 .Based on the difference of the mRNA and the KEGG enrichment analysis,the expression of PPAR signal pathway was the highest in the castrated pigs,of which fourteen genes were upregulated and three genes were downregulated.Lipogenic genes like fatty acid binding protein 7 gene(FABP7),aquaporin 7 gene(AQP7),retinoid X receptor,gamma gene(RXRγ)were upregu-lated significantly in castrated pigs,and lipolysis genes were downregulated like phosphoenolpyruvate carboxykinase 1 gene(PCK1),peroxisome proliferator-activated receptor gamma,coactivator 1 alpha gene(PPARGC1α)and cyto-chrome P450,family 7,subfamily A,polypeptide 1 gene(CYP7A1).