| 不同种（品种）三叶草愈伤组织诱导及分化影响因素研究 |
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| 引用本文： | 梁月香,方敏彦,梁慧敏,袁庆华,王小春.不同种（品种）三叶草愈伤组织诱导及分化影响因素研究[J].山东林业科技,2011,41(5):1-5,63. |
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| 作者姓名： | 梁月香 方敏彦 梁慧敏 袁庆华 王小春 |
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| 作者单位： | 1. 江苏农林职业技术学院农艺系,江苏句容,212400
2. 中国农业科学院北京畜牧兽医研究所 |
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| 基金项目： | 江苏省高技术研究项目，“十二五”农村领域国家科技计划课题，江苏省“农业三新工程”项目(sx |
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| 摘 要： | 以9个三叶草品种的上胚轴、下胚轴、叶片和子叶为外植体，研究了影响三叶草愈伤组织诱导和再分化的主要因素。结果表明，不同品种不同外植体愈伤诱导率差异较大，下胚轴为较适宜的外植体，其平均愈伤组织诱导率和愈伤分化率为97％和10．3％，改良SH＋2，4-D8．0mg／L＋6-BA0．2mg／L＋KT0．2mg／L为最佳胚性愈伤组织诱导培养基，所形成的愈伤组织的愈伤分化率也最高。
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| 关 键 词： | 三叶草 外植体 胚性愈伤组织 愈伤分化 |
A Study of ImpaCt Factors on Induction and Regeneration of Callus Tissues in Different Cultivars of Clover |
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| Liang Yuexiang,Fang Minyan,Liang HuiminYuan Qinghua,Wang Xiaochun.A Study of ImpaCt Factors on Induction and Regeneration of Callus Tissues in Different Cultivars of Clover[J].Journal of Shandong Forestry Science and Technology,2011,41(5):1-5,63. |
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| Authors: | Liang Yuexiang Fang Minyan Liang HuiminYuan Qinghua Wang Xiaochun |
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| Institution: | Liang Yuexiang1,2,Fang Minyan1,Liang Huimin1Yuan Qinghua3,Wang Xiaochun1(1.Agriculture Department of Jiangsu Agriculture and Forestry Profession Technology College,Jurong 212400,Jiangsu China,2.Ningxia Academy of Agriculture and Forestry,3.Institute of Animal Sciences,Chinese Academy of Agricultural Sciences) |
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| Abstract: | The Hypocotyls,The Upside,Downside, Leaf and Cotyledon of Nine Varieties of Clover（Trifolum）were Used to Study the Effect of Hormone Concentrations on Callus Induction and Redifferentiation. Different Varieties and Explants Each had Different Induction Requirements. The Downside of Hypocotyl Callus Induction Average Rate of Clover was 97%,While That of Differential Ratio was 10. 3%. The Best Medium for Embryo Genie Callus Induction and Callus Differentiation was Modified SH-2,4-D 8.0 mg/L＋6-BA0.2 mg/L＋KT0.2 mg/L. |
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| Keywords: | Clover Explants Embryogenic callus Callus Differentiation |
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