稳定性肥料中硝化抑制剂作用效果的检测方法

以硝化抑制率作为评价指标,研究了影响硝化抑制剂抑制效果测定方法的主要因素,包括氮土比、培养时间以及土壤类型等。确立了测定稳定性肥料中硝化抑制剂抑制作用效果的最佳检测方法:称取风干后的棕壤200 g,以氮土比1.15∶1 000准确称取样肥,并将其充分混匀,以25%的含水量,在30℃培养箱中培养。选择自培养开始的第9、12、15 d测定土壤中硝态氮(包括亚硝态氮)的质量分数。该检测方法提高了评价效率和准确度。

solid phase extraction(SPE);high performance liquid chromatography tandem mass spectrometry(HPLC MS/MS);antibiotics residues;organic fertilizer

Based on the nitrification inhibitory rate as evaluating indicator,the factors that affect the inhibiting efficiency evaluation of nitrification inhibitor were studied,which including:soil nitrogen ratio,incubation time and soil types and etc.A rapid detection method was established for evaluating the efficiency of nitrification inhibitor in stabilized fertilizers:200 g of dried brown soil were weighed,and then the amount of fertilizer were weighed according to the nitrogen and soil ratio at 1.15: 1 000.The mixed soil and fertilizer were cultured in the incubator at 30 ℃with 25％the water content.Soil nitrate(including nitrite)content was measured at the 9,12,15 day.The detection method increased the evaluating efficiency and accuracy.