普通菜豆抗炭疽病基因SCAR标记鉴定

利用12个菜豆品种（鉴别寄主）评价了7个抗炭疽病基因SCAR标记的可靠性和实用性，其中SBB14_1150/1050标记引物扩增没有特异性，SAS13₉₅₀没有扩增带。用5个可靠的菜豆抗炭疽病基因SCAR标记（SCAreoli₁₀₀₀、SH18₁₁₀₀、SAB3₄₀₀、SB12₃₅₀ 和SCF10₁₀₇₂），对127份普通菜豆抗炭疽病品种进行抗炭疽病基因分子标记鉴定，82份未检测到SCAR标记，45份分别含有1~3个SCAR标记；检测到SCAR标记的资源中，13份含有SCAreoli₁₀₀₀标记，13份含有SH18₁₁₀₀标记，5份含有SAB3₄₀₀标记，9份含有SB12₃₅₀标记，11份含有SCF10₁₀₇₂标记。分析表明抗病品种含有的抗病基因标记与品种来源存在相关性。

Identification of Anthracnose Resistant Genes Based on SCAR Markers in Common Bean (Phaseolus vulgarisL.)

Bean Anthracnose is one of the main diseases,which causes 20%-30% reduction of output in common bean.Based on the evaluated results of disease resistance to anthracnose with No.81 physiological race from 1986 to 2000,127 accessions of resistant and high resistant germplasm resources were selected to be used in this study.In order to identify different types of resistance genes in these materials,we assessed the reliability and effectiveness of 7 SCAR primers(SBB141150/1050,SAS13950,SCAreoli1000,SH181100,SAB3400,SB12350,and SCF101072)which linked with resistant anthracnose genes.12 identification host varieties of common bean were used as control.The results showed that SBB141150/1050 had no differential amplification and SAS13950 had no target amplification.The further identification of anthracnose resistant genes was conducted by using 5 SCAR primers in 127 accessions of common bean.There were no SCAR markers detected in 82 accessions.1-3 SCAR markers appeared in 45 accessions respectively.SCAreoli1000(Co-2) marker appeared in 13,SH181100(Co-42) marker in 13,SAB3400 (Co-5) marker in 5,SB12350(Co-9) marker in 9,SCF101072(Co-10) marker in 11 accessions of common bean.The results also revealed that there were 5 accessions with broad-spectrum resistance to anthracnose and there was a correlation between the resistant genes and origin of the germplasm resource.This study provided an important genetic information for breeding program on disease resistance in common bean.