| 抗真菌α-硫堇蛋白DB_4基因导入苹果的研究 |
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| 引用本文： | 孙春玉,孙旸,魏海蓉,宗晓娟,刘庆忠.抗真菌α-硫堇蛋白DB_4基因导入苹果的研究[J].安徽农业科学,2010,38(3):1147-1148. |
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| 作者姓名： | 孙春玉 孙旸 魏海蓉 宗晓娟 刘庆忠 |
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| 作者单位： | 山东省果树研究所山东省果树生物技术育种重点实验室,山东泰安,271000;吉林农业大学生命科学学院,吉林长春,130118;吉林农业大学生命科学学院,吉林长春,130118;山东省果树研究所山东省果树生物技术育种重点实验室,山东泰安,271000 |
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| 基金项目： | 国家863计划项目(2008AA10Z157) |
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| 摘 要： | ［目的］利用转基因的办法提高苹果（Malus domestica Borkh）对叶部病害和幼果期侵染果实病害的抵抗能力。［方法］利用农杆菌介导的遗传转化方法将绿色组织特异表达的菠菜核酮糖二磷酸羧化酶/加氧酶的激活酶（Rubisco activase）启动子（RCAP）驱动下的α堇蛋白DB4基因转入到“皇家嘎啦”苹果叶片外植体中。［结果］ PCR、Southern blotting分析及GUS检测结果表明，目的基因已经整合到苹果基因组上。［结论］获得了转α堇蛋白DB4基因的转基因苹果。
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| 关 键 词： | α-硫堇蛋白 抗真菌 转基因苹果 |
Study on the Transgenic Royal Gala Apple (Malus domestic Borkh) with Antifungal a-thionin DB_4 Gene |
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| SUN Chun-yu et al.Study on the Transgenic Royal Gala Apple (Malus domestic Borkh) with Antifungal a-thionin DB_4 Gene[J].Journal of Anhui Agricultural Sciences,2010,38(3):1147-1148. |
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| Authors: | SUN Chun-yu |
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| Institution: | Key Laboratory for Fruit Biotechnology Breeding of Shandong Province;Shandong Institute of Pomology;Taian;Shandong 271000 |
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| Abstract: | Objective] The research aimed to improve the resistance to leaf plant disease of apple(Malus domestic Borkh) by transgenic method. Method] By using Agrobacterium-mediated transformation method, the putative transgenic "Royal Gala" apple with antifungal α-thionin DB_4 gene which driven by green specific spinach ribulose bisphosphate carboxylase/oxygenase(rubisco) activase promoter(RCAP) was obtained. Result] PCR, Southern blotting analysis and GUS assay confirmed that α-thionin DB_4 gene had been integrated into "Royal Gala" apple. Conclusion] The transgenic apple integrated with antifungal α-thionin DB_4 gene was obtained. |
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| Keywords: | Antifungus Transgenic apple |
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