尿素对葡萄5 个氮代谢相关基因表达的影响

 以‘藤稔’葡萄为试材，利用半定量RT-PCR 和荧光定量PCR 技术对5 个葡萄氮代谢基因 VvGHD（JF796045）、VvNiR（JF796046）、VvNR（JF796047）、VvGS（JF796048）和VvAS（JF796049） 在施用尿素后的表达情况进行了分析。5 个基因在葡萄叶面喷施尿素后不同时间段的表达量存在显著差 异，整体呈先升高后降低的趋势；VvGDH 最高表达水平发生在尿素喷施后48 h，VvNR 和VvNiR 最高表 达水平发生在24 h，VvGS 最高表达水平发生在6 h，VvAS 最高表达水平发生在2 h。叶面喷施比土壤施用 尿素叶中5 个基因表达高峰出现的时间早，且表达水平高；土壤施尿素后叶中5 个基因对尿素的响应时 间比根中延后，但响应强度明显高于根中。

Effects of Fertilizer Application on Expression of Genes Related to Nitrogen Metabolism in Fujiminori Grapevine

To understand gene response to fertilizer application，quantitative PCR and semiquantitative PCR were used to analyze the expression of five genes（VvGHD，VvNiR，VvNR，VvGS and VvAS）of‘Fujiminori’grape at different times with the fertilizer application. The results show that the expression levels of five genes exhibit significant differences at different times after treatment，the overall trend was first increased and then decreased；The highest expression level of VvGDH was in 48 h，VvNR and VvNiR the highest expression level in 24 h，VvGS the highest expression level in 6 h，VvAS the highest expression level in 2 h. The highest expression levels of five genes after ground fertilization in leaves were delayed longer than those after foliar fertilizer application，and the overall expression level lower than foliar fertilizer application；Response of five genes after ground fertilization in leaves was delayed longer than the root，but the response intensity was significantly higher than corresponding genes in the root.