4种中药成分对犬瘟热模型犬支气管平滑肌细胞Kv通道的作用

应用细胞膜片钳技术，分别对正常对照组、犬瘟热（CD）组、川芎嗪组、黄芩甙组、黄芪组、黄连素组犬支气管平滑肌细胞（BSMC）电压门控性钾通道（Kv）动力学指标、静息膜电位（Em）、静息膜电容（Cm）和电流密度（pA／pF）进行了测定。结果表明，CD组BSMC膜Kv活性显著降低。川芎嗪、黄芩甙、黄芪3种中药（成分）均能不同程度拈抗CD模型犬BSMC膜Kv通道活性的抑制，促进支气管平滑肌细胞膜Kv通道的开放。黄芩甙具有显著提高支气管平滑肌细胞膜Kv通道活性，促进支气管平滑肌舒张作用，在4种中药（成分）中表现突出。

Effects of Four Chinese Herbal Medicinal Ingredients on Voltage-gated K+ Channels of Bronchial Smooth Muscle Cells from Canine Distemper Dogs

The voltage-gated K~ channels dynamical indices,resting membrane potential and potassium currents of bronchial smooth muscle cells(BSMC) were investigated by cell patch clamp technique.The experimental BSMCs of dogs were divided into control group,CD group,Tetramethylpyazine group,Baicalin group,Astraglus membranaceus group and Berberine group.The results indicated that the activation of Kv channel from CD group was significantly inhibited.This inhibitation was counteracted by Tetramethylpyazine,Baicalin and Astraglus membranaceus,which made the Kv channel of BSMC open.The most powerful one of four Chinese herbal medicinal ingredients was Baicalin.Baicalin increased the activation of the Kv channel of BSMC from CD dogs significantly and extended bronchial smooth muscle cells.