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Monoclonal antibody-based immunodot for evaluating serum immunoglobulin levels in rohu (Labeo rohita) |
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| Authors: | Padinhate Purayil Suresh Babu Kalkuli Mariappa Shankar Badami Ramalingappa Honnananda Purandara Ballyaya Abhiman |
| Institution: | 1. Central Institute of Fisheries Education, Kakinada Centre, Near Old Burma Shell, Beach Road, Kakinada, 533007, Andhra Pradesh, India 2. Fish Pathology and Biotechnology Laboratory, Department of Aquaculture, College of Fisheries, Mangalore, 575002, Karnataka, India 3. Department of Aquaculture, College of Fisheries, Chhattisgarh Kamdhenu Vishwavidyalaya, Kawardha, Kabirdham, 491995, India |
| Abstract: | Immune response in rohu treated with an immunomodulator is usually evaluated employing either non-specific immune parameters or traditional antibody-based tools. In the present study, a monoclonal antibody-based immunodot has been developed for evaluating antibody titre in rohu as a preliminary tool to ensure antibody response due to the effect of an immunomodulator, which can be used for routine field level analysis. The immunodot was sensitive enough to determine rohu immunoglobulin up to 15 μg/ml. Application of the immunodot for evaluating enhancement in immune response could be successfully demonstrated in probiotic fed rohu. |
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