除虫菊愈伤组织的诱导和继代

以除虫菊茎尖为外植体,MS培养基为基本培养基,对除虫菊愈伤组织的诱导和继代进行了系统研究。结果表明:外源激素2,4-D与KT的9种配比中,1.0与0.4mg/L的激素组合对除虫菊愈伤组织的诱导率可达88.0%;当蔗糖质量浓度为10～40g/L时,蔗糖质量浓度的大小与愈伤组织增长量成正比,与褐变开始时间呈负相关关系,当质量浓度为25g/L时,愈伤组织日平均生长量可达0.127g;适宜的光照时间可延长愈伤组织保存期并维持良好的组织结构,光照时间为4h/d时,愈伤组织褐化开始时间保持在27d左右。

Induction,preservation and differentiation of calli of Pyrethrum cinerariifolium Trev.

With stem tip as explants and MS as basic culture medium,studies were carried out on continuing growth and induction of subculture of Pyrethrum cinerariifolium Trev..The results show that:among the nine different treatments with the combinations of 2,4-D and KT,the better one is 1.0 mg/L vs 0.4 g/L.By doing so,we can get an ideal induction rate 88.0%;when the concentration of sucrose is ranged from 10 to 40 g/L,it will be in the direct proportion with increment of callus,while with the inverse proportion with start time of callus browned.Especially when the concentration of sucrose is 25 g/L,the callus increment can get to 0.127 g/d;at the same time,proper illumination can prolong the preserving time and maintain an upper tissue structure.If it being illuminated by 4 h/d,the beginning time of being browned of callus will exceed 27 days.