茉莉酸甲酯和生长素对苦玄参苷积累的影响

【目的】研究植物生长调节物质对人工种植苦玄参中苦玄参苷积累的影响,为苦玄参人工种植化学调控措施的制定提供参考。【方法】采用不同浓度的茉莉酸甲酯(MeJA)和生长素(IAA)对苦玄参植株进行叶面喷施和浸根处理,测定其苦玄参苷IA、IB及苦玄参总苷积累量。【结果】MeJA叶面喷施和浸根处理均以20 mg/L对苦玄参苷IA的调控效果最佳,此时苦玄参苷IA积累量最高,分别达0.63%和0.62%,均显著高于清水对照(CK)(P<0.05,下同)。2 mg/L MeJA叶面喷施对苦玄参苷IB的促进作用最强,随MeJA浓度增加其促进作用减弱;浸根处理时,随MeJA浓度增加其促进作用逐渐加强,至20 mg/L时苦玄参苷IB积累量达峰值0.34%。两种处理方式下,苦玄参总苷积累量均在MeJA浓度为20 mg/L时最高,叶面喷施整体优于浸根处理。 IAA叶面喷施时,随其浓度增加,苦玄参苷IA积累量先降低后升高,浓度为100 mg/L时苦玄参苷IA积累量最高,达0.68%,显著高于CK和其他浓度处理;浸根处理时,苦玄参苷IA积累量的变化趋势与叶面喷施相反,浓度为10 mg/L时促进作用最强,随IAA浓度增加苦玄参苷IA积累量逐渐降低。两种处理方式均以IAA 50 mg/L对苦玄参苷IB的促进作用最强。 IAA对苦玄参总苷积累量的影响与其对苦玄参苷IA的影响趋势相似,叶面喷施时100 mg/L的促进作用最强,浸根处理则以50 mg/L时苦玄参总苷积累量最高。两种处理方式相比,IAA浸根处理优于叶面喷施。【结论】MeJA和IAA均可通过叶面喷施和浸根处理促进苦玄参苷的积累,其中,MeJA宜选择20 mg/L进行叶面喷施, IAA宜选择50 mg/L进行浸根处理。

Effects of methyl jasmonate and indole-3-acetic acid on accumulation of picfeltarraenin in Picria felterrae Lour

Objective]The present study was conducted to investigate the impact of methyl jasmonate(MeJA) and in-dole-3-acetic acid(IAA) on accumulation of picfeltrarraenins in planted Picria felterrae Lour. individuals, in order to provide a reference for development of chemical regulation measures for planted individuals of the species. Method]Differ-ent concentrations of MeJA and IAA solutions were sprayed onto leaf surface and soaked roots of P. felterrae Lour. individu-als, and accumulated amount of picfeltrarraenin IA and IB and total picfeltrarraenins were measured. Result]When the concentration of MeJA solution was 20 mg/L, the strongest regulative effect on picfeltrarraenin IA was achieved for both fo-liage spray and root soaking, the accumulative amount of picfeltrarraenin IA reaching the maximum levels, 0.63% and 0.62%respectively, significantly higher than that induced by clean water control(CK)(P<0.05, the same below). MeJA at 2 mg/L showed the strongest stimulative effect on accumulation of picfeltrarraenin IB, and this effect became weakened as the con-centration increased; as regards root soaking, the promoting effect strengthened with increase in MeJA concentration, and accumulative amount of picfeltrarraenin IB reached the highest level 0.34% when concentration was 20 mg/L. The accumula-tive amount of total picfeltrarraenins reached the highest level when the MeJA concentration was 20 mg/L for both treat-ments, the overall effect of foliage spray was better than that of root soaking. For foliage spray with IAA solution, accumula-tive amount of picfeltrarraenin IA decreased firstly and then increased as concentration of IAA solution increased, and reached the maximum level 0.68% at 100 mg/L which was significantly higher than that induced by other concentrations and CK. For root soaking with IAA solution, accumulation of picfeltrarraenin IA showed a reverse trend relative to that for foliage spray, and stimulative effect of IAA reached the maximum level at 10 mg/L of IAA, accumulation of picfeltrarraenin IA dropped as IAA concentration increased. Both foliage spray and root soaking showed the strongest stimulative effect on accu-mulation of picfeltrarraenin IB at 50 mg/L of IAA. The effect of IAA on total picfeltrarraenins accumulation was similar to its effect on picfeltrarraenin IA, the stimulative effect reaching the highest level(i.e. the content of total picfeltrarraenins accu-mulation was at its peak) at 100 mg/L for foliage spray and at 50 mg/L for root soaking. Root soaking with IAA solution was better than foliage spray. Conclusion]Both MeJA and IAA can stimulate accumulation of picfeltrarraenins via foliage spray or root soaking. The best stimulative effect may be achieved when 20 mg/L of MeJA is used through foliage spray or 50 mg/L of IAA via root soaking.