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| 猪瘟病毒E2基因的克隆表达 | |
| 引用本文： | 朱文豪,张以芳,徐引弟,王治方,张玉杨,郭成留.猪瘟病毒E2基因的克隆表达[J].河南农业科学,2009(9). |
| 作者姓名： | 朱文豪 张以芳 徐引弟 王治方 张玉杨 郭成留 |
| 作者单位： | 1. 河南省农业科学院,畜牧兽医研究所,河南,郑州,450002;云南农业大学,动物科技学院,云南,昆明,650201 2. 云南农业大学,动物科技学院,云南,昆明,650201 3. 河南省农业科学院,畜牧兽医研究所,河南,郑州,450002 |
| 基金项目： | 国家自然科学基金项目，河南省农科院专项发展基金 |
| 摘 要： | PCR扩增猪瘟病毒(CSFV)定点突变后的E2基因,克隆至原核表达载体质粒pET-28a中,重组质粒pET28a-E2转化BL21(DE3),IPTG诱导,高效表达了E2基因,表达量达菌体总蛋白的25.3%。免疫印迹表明,所表达的蛋白是CSFV特异性的。 |
| 关 键 词： | 猪瘟病毒 E2基因 原核表达 |
Cloning and Expression of E2 Gene of Classic Swine Fever Virus |
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| ZHU Wen-hao,ZHANG Yi-fang,XU Yin-di,WANG Zhi-fang,ZHANG Yu-yang,GUO Cheng-liu.Cloning and Expression of E2 Gene of Classic Swine Fever Virus[J].Journal of Henan Agricultural Sciences,2009(9). | |
| Authors: | ZHU Wen-hao ZHANG Yi-fang XU Yin-di WANG Zhi-fang ZHANG Yu-yang GUO Cheng-liu |
| Institution: | 1.Institute of Animal Husbandry and Veterinary Research;Henan Academy of Agricultural Sciences;Zhengzhou 450002;China;2.College of Animal Science and Technology;Yunnan Agricultural University;Kunming 650201;China |
| Abstract: | A mutated CSFV E2 gene was amplified by PCR and cloned into pET-28a to construct the prokaryotic expression vector pET28a-E2.The recombinant plasmid was transformed into BL21(DE3) and expressed at high level induced by IPTG.The expressed E2 protein was amounted to 25.3% of the total bacteria proteins.The result of Western-blot showed that the recombinant E2 protein was CSFV-specific. |
| Keywords: | CSFV E2 gene Prokaryotic expression |
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