In vivo staining of reduced iron by 2,2′ bipyridine in rice exposed to iron toxicity |
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| Authors: | Katrin Engel Folkard Asch Mathias Becker |
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| Affiliation: | 1. Institute of Crop Science and Resource Conservation, University of Bonn, Germany;2. Institute of Plant Production and Agroecology in the Tropics and Subtropics, University of Hohenheim, Germany |
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| Abstract: | A protocol for a novel method to visualize Fe(II) in rice tissues is proposed. The method is based on the selective formation of a purple‐red color complex of 2,2′ bipyridine and Fe(II). Rice genotypes were exposed to 18 mM Fe(II) in nutrient solution for 2 d. Root systems of intact plants were subsequently placed in 2,2′ bipyridine solutions. The formation of the [Fe(bipy)<$>_3^{2+}<$>] color complex was visualized using bifocal microscopy. The method may improve the selection of genotypes during breeding for Fe‐toxicity resistance of rice. |
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| Keywords: | bifocal microscopy DPD leaf bronzing Oryza sativa L. selection |
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