| 分子标记辅助培育双抗稻瘟病和白叶枯病杂交稻恢复系 |
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| 引用本文： | 陈建民,付志英,权宝权,田大刚,李刚,王锋.分子标记辅助培育双抗稻瘟病和白叶枯病杂交稻恢复系[J].分子植物育种,2009,7(3). |
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| 作者姓名： | 陈建民 付志英 权宝权 田大刚 李刚 王锋 |
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| 作者单位： | 1. 福建省农业科学院生物技术研究所,福州,350003
2. 福建农业职业技术学院,福州,350007 |
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| 基金项目： | 福建省科技重大专项基金，福建省科技厅重点项目 |
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| 摘 要： | 本研究利用常规回交育种结合分子标记辅助选择技术,将来自C750的抗白叶枯病基因Xa23和抗稻瘟病基因pi9聚合到感病杂交稻恢复系闽恢3139中.最后在回交后代中获得了4个双抗稻瘟病和白叶枯病改良系ZR21-sk1、ZR21-sk2、ZR21-sk3和ZR21-sk4,且其遗传背景恢复率达96%以上.用来自福建省具有代表性的24个稻瘟病菌株和白叶枯病广致病型菌系P6对改良株系进行人工接菌鉴定,结果表明,聚合了Xa23和pi9基因的改良系同时抗稻瘟病和白叶枯病,且抗性与抗谱与供体亲本C750相似.农艺性状分析显示,改良株系所配组合基本保持闽恢3139的农艺性状和配合力,可直接应用于生产或作抗性育种亲本.
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| 关 键 词： | 分子标记辅助选择 稻瘟病 白叶枯病 杂交稻恢复系 pi9/Xa23基因 |
Breeding Hybrid Rice Restoring Line with Double Resistance to Rice Blast and Bacterial Blight by Marker-Assisted Selection |
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| Chen Jianmin,FuZhiying,QuanBaoquan,TianDagang,LiGang,WangFeng.Breeding Hybrid Rice Restoring Line with Double Resistance to Rice Blast and Bacterial Blight by Marker-Assisted Selection[J].Molecular Plant Breeding,2009,7(3). |
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| Authors: | Chen Jianmin FuZhiying QuanBaoquan TianDagang LiGang WangFeng |
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| Institution: | Chen Jianmin 1 Fu Zhiying 2 Quan Baoquan 1 Tian Dagang 1 Li Gang 1 Wang Feng 1 1 Biotechnology Research Institute,Fujian Academy of Agricaltural Sciences,Fuzhou,350003,2 Fujian Agricultural Vocation-Technical College,350007 |
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| Abstract: | Through molecular marker-assisted selection (MAS) and traditional breeding methods, the blast resistance gene pi9 and bacterial blight (BB) resistance gene Xa23 from C750 were introduced into the susceptible restorer line Minhui3139. Four improved lines ZR21-sk1, ZR21-sk2, ZR21-sk3 and ZR21-sk4 which conferred double homologous resistance genes and good agronomic characteristics were obtained through molecular marker test for the R target genes and different parental genetic background analysis. By artifici... |
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