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靛酚蓝-分光光度法测定胃肠道内容物中氨态氮含量研究
引用本文:蒲万霞,魏云霞,孟晓琴,王玲,陈炅然,杨志强. 靛酚蓝-分光光度法测定胃肠道内容物中氨态氮含量研究[J]. 甘肃农业大学学报, 2008, 43(5)
作者姓名:蒲万霞  魏云霞  孟晓琴  王玲  陈炅然  杨志强
作者单位:1. 甘肃农业大学动物医学院,甘肃,兰州,730070;中国农业科学院兰州畜牧与兽药研究所,甘肃,兰州,730050
2. 中国农业科学院兰州畜牧与兽药研究所,甘肃,兰州,730050
3. 甘肃农业大学动物医学院,甘肃,兰州,730070
基金项目:中央级公益性科研院所基本科研业务费专项基金
摘    要:
对靛酚蓝-分光光度法检测胃肠道内容物中氮含量的试验条件进行了筛选,结果表明:催化剂质量浓度25 mg/L,在640 nm的波长下,40℃水溶显色20 min为最佳检测试验条件;氨态氮标准曲线相关系数为0.999 1,线性范围为0~5.0μg,摩尔吸光系数ε640=1.26×103 L/(mol.cm),最低检出限为0.127μg;该方法与比色法的测定结果间无显著性差异,可用于胃肠道内容物中氨态氮含量的检测.

关 键 词:靛酚蓝-分光光度法  胃肠道内容物  氨氮

Determination of NH+4-N content in castrointestinal with indophenol blue spectrophotometric method
PU Wan-xia,WEI Yun-xia,MENG Xiao-qin,WANG Ling,CHEN Jiong-ran,YANG Zhi-qiang. Determination of NH+4-N content in castrointestinal with indophenol blue spectrophotometric method[J]. Journal of Gansu Agricultural University, 2008, 43(5)
Authors:PU Wan-xia  WEI Yun-xia  MENG Xiao-qin  WANG Ling  CHEN Jiong-ran  YANG Zhi-qiang
Abstract:
The impact factors of determination of NH+4-N content in gastrointestinal contents were screened with indophenol blue spectrophotometric method.The results showed that the optimum factors were reaction temperature at 40 ℃,reaction time for 20 min,catalyst at 25 mg/L and determinate at 640 nm,respectively.A linear work curve of NH+4-N with R2 = 0.999 1 had been achieved,with 0~5.0 μg of linearity extension and ε640=1.26×103 L/(mol·cm).No significant difference in determination results was found between this method and colorimetry method.It was suggested that indophenol blue spectrophotometric method can be used as a method to determine NH+4-N content in gastrointestinal contents.
Keywords:indophenol blue spectrophotometric method  gastrointestinal content  ammonia nitrogen
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