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春兰‘金荷鼎’ב赤蕙’杂交根状茎芽分化及生根的研究
引用本文:牛田,张林,杨凯,李承秀,于永畅,颜婷美,聂硕,朱翠翠,王长宪.春兰‘金荷鼎’ב赤蕙’杂交根状茎芽分化及生根的研究[J].中国农学通报,2014,30(13):252-255.
作者姓名:牛田  张林  杨凯  李承秀  于永畅  颜婷美  聂硕  朱翠翠  王长宪
作者单位:1. 山东省泰山林业科学研究院2. 3. 山东农业大学林学院4. 泰山林业科学研究院5. 山东农业大学6. 泰山林业科学院
基金项目:国兰新品种培育与产业化生产关键技术研究
摘    要:以‘金荷鼎’ב赤蕙’根状茎为材料,研究根状茎芽分化和生根情况,包括BA与IBA的浓度配比对分化的影响和BA与NAA的浓度配比对生根的影响。结果表明,1/2MS+IBA 0.2 mg/L+BA 2 mg/L为春兰‘金荷鼎’ב赤蕙’根状茎的最佳分化培养基;春兰杂交兰最佳生根培养基为1/2MS+BA0.1 mg/L+NAA 2 mg/L。杂交育种与组织培养技术结合是国兰新品种选育的有效方法。

关 键 词:春兰  根状茎  培养基
收稿时间:2013/10/23 0:00:00
修稿时间:2013/11/14 0:00:00

Study on Budinitiation and Rooting of Cymbidium goeringii‘ Jinheding' and ‘ Chihui'
Institution:Niu Tian, Zhang Lin, Wang Houxin, Li Chengxiu, Yu Yongchang,Yan Tingmei, Nie Shuo, Zhu Cuicui, Wang Changxian (1. College of Forestry, Shandong Agriculture University, Tai'an Shandong 271018; 2.Taishan Forestry Academe, Tai' an Shandong 271000)
Abstract:Taking‘Jinheding'and‘Chihui'as parents, rootstocks were used as materials for studying the budinitiation and rooting of rootstocks. There were including the effects of BA and IBA on the bud initiation andthe effects of BA and NAA on the rooting. The result showed that 1/2MS+ IBA 0.2 mg/L+ BA 2 mg/L was thebest medium for bud initiation and 1/2MS+ BA 0.1 mg/L+ NAA 2 mg/L was the best medium for rooting. Crossbreeding technology combining with tissue culture was the effective way for the new species breeding ofCymbidium.
Keywords:medium
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