‘蕲山药’离体再生及试管微块茎的形成

为解决生产上‘蕲山药’脱毒种药供不应求的问题，以其茎节为试验材料，研究不同植物生长调节剂配比对‘蕲山药’不定芽再生的影响以及不同碳源对试管微块茎形成的影响，建立‘蕲山药’的离体再生体系及高效形成试管微块茎的方法。结果表明，在不同植物生长调节剂配比对‘蕲山药’诱导不定芽分化的影响中，MS＋6-BA0．5mg／L＋NAA0．1mg／L或MS＋ZT0．2mg／L＋NAA0．2mg／L对‘蕲山药’不定芽分化较好，形成不定芽频率分别达到81．33％、82．67％；MS＋6-BA0．2mg／L＋NAA0．02mg／L对‘蕲山药’不定芽增殖效果最好，增殖系数高达4．7；Ms＋NAA0．02mg／L适合‘蕲山药’不定芽生根，生根率高达96．32％。150mg／LPvP能有效降低‘蕲山药’组织培养的褐化现象。试验还发现，MS＋BA0．5mg/L＋NAA0．05mg／L＋60g／L蔗糖，最有利于‘蕲山药’试管微块茎的形成与生长，其微块茎形成率最高，达到了97．50％；其60、120天微块茎质量分别达到了0．18g和0．39g。

A Rapid in Vitro Regeneration System and the Formation of in Vitro Micro Tubers of Dioscorea opposita ‘ Qi ＇

In order to solve the shortage of materials, the ratio of different plant growth virus-free seed yam production, with Its stem sechon as the test regulators on the impact of ‘ Qi＇ yam in vitro shoot regeneration as well as the influence of different carbon sources on the in vitro micro tuber formation were studied. The ‘Qi＇ yam efficient in vitro shoot regeneration system and in vitro production method of micro tubers were established. The results showed that it was useful for‘Qi＇ yam adventitious bud regeneration in the MS media with 0.5 mg/L 6- BA and 0.1 mg/L NAA or with 0.2 mg/L ZT and 0.2 mg/L NAA, and the ratio of ‘ Qi＇ yam adventitious bud differentiation reached 81.33% and 82.67%, respectively. In the MS media with 0.2 mg/L 6- BA and 0.02 mg/L NAA, ‘ Qi＇ yam adventitious bud multiplication was effective and multiplication factor was as high as 4.7. The MS media with 0.02 mg/L NAA was suitable for the root growth of the ‘Qi＇ yam plants, and the rooting rate was reached 96.32%. 150 mg/L PVP could effectively reduced the brown phenomenon in the ‘Qi＇ yam tissue culture. The production method of in vitro micro tubers of Dioscorea opposita ‘Qi＇ had also been established. The most favorable formation rate of ‘Qi＇ yam micro tuber was gained in the MS media with 0.5 mg/L 6-BA, 0.05 mg/L NAA and 60 g/L sucrose. In these conditions, the highest micro tuber formation rate was 97.50%, and the micro tuber weight reached 0.18 g and 0.39 g after 60 days and 120 days, respectively.