| 苹果全基因组SBP-box基因家族分析及代表成员的分子克隆 |
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| 引用本文： | 张晓辉,魏小春,李锡香,孙玉燕,王 冠,常兆晶,刘冠群,邱 杨,宋江萍,王海平,沈 镝,王大江,韩月澎.苹果全基因组SBP-box基因家族分析及代表成员的分子克隆[J].园艺学报,2014,41(2):215-226. |
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| 作者姓名： | 张晓辉 魏小春 李锡香 孙玉燕 王 冠 常兆晶 刘冠群 邱 杨 宋江萍 王海平 沈 镝 王大江 韩月澎 |
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| 作者单位： | （1中国农业科学院蔬菜花卉研究所，农业部园艺作物生物学与种质创制重点实验室，北京 100081；2中国农业科学院果树研究所，辽宁兴城 125100；3中国科学院武汉植物园，武汉 430074） |
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| 摘 要： | 从金冠苹果基因组中鉴定出33个SBP-box家族基因，其中20个为miR156的靶基因。通过进化分析，对该家族成员进行了系统分类和功能预测。大部分预测的基因在富士苹果基因组中存在并表达，并且发现可变剪切在该家族基因中高频出现。在富士苹果中克隆了4个代表性成员基因，即MdSPL2、 MdSPL11、MdSPL13和MdSPL33；与金冠相比，MdSPL33是一个缺失第2和3外显子的剪切本，并具有2个单碱基变异；MdSPL11和MdSPL13分别存在4个和2个单碱基变异，造成3个和1个氨基酸改变。
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| 关 键 词： | 苹果 SBP-box家族 分子进化 基因克隆 |
| 收稿时间： | 2013-09-24 |
Genome-wide Analysis of SBP-box Gene Family and Molecular Cloning of Its Typical Members in Malus × domestica |
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| ZHANG Xiao-hui,WEI Xiao-chun,LI Xi-xiang,SUN Yu-yan,WANG Guan,CHANG Zhao-jing,LIU Guan-qun,QIU Yang,SONG Jiang-ping,WANG Hai-ping,SHEN Di,WANG Da-jiang,HAN Yue-peng.Genome-wide Analysis of SBP-box Gene Family and Molecular Cloning of Its Typical Members in Malus × domestica[J].Acta Horticulturae Sinica,2014,41(2):215-226. |
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| Authors: | ZHANG Xiao-hui WEI Xiao-chun LI Xi-xiang SUN Yu-yan WANG Guan CHANG Zhao-jing LIU Guan-qun QIU Yang SONG Jiang-ping WANG Hai-ping SHEN Di WANG Da-jiang HAN Yue-peng |
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| Institution: | （1Institute of Vegetables and Flowers，Chinese Academy of Agricultural Sciences；Key Laboratory of Biology and Genetic Improvement of Horticultural Crops，Ministry of Agriculture，Beijing 100081，China；2Research Institute of Pomology，Chinese Academy of Agricultural Sciences，Xingcheng，Liaoning 125100，China；3Wuhan Botanical Garden，Chinese Academy of Sciences，Wuhan 430074，China） |
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| Abstract: | The present study isolated 33 SBP-box family genes including 20 miR156 targets in Golden Delicious apple genome. The phylogenic analyses were performed and the biological functions were predicted based on the phylogenic tree. The existence and expression pattern of SBP-box family genes were investigated by PCR and RT-PCR analysis. Alternative splicing events were commonly found in this gene family. Four typical members，MdSPL2，MdSPL11，MdSPL13 and MdSPL33 were cloned from Fuji apple tree. Compared to Golden Delicious cultivar，MdSPL33 from Fuji produced an alternative splicing version which lost the 2nd and 3rd exons and harboring two single base mutations. MdSPL11 and MdSPL13 have four and two single base mutations causing three and one amino acid changes，respectively. |
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| Keywords: | Malus× domestica SBP-box family phylogenetic gene cloning |
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