高粱种子硫酸二乙酯诱变处理的研究

为了探明化学诱变剂DES诱变处理效应，开展了高粱不同基因型、不同DES浓度和不同处理时间的比较试验。通过同一品种、相同时间、不同DES浓度比较试验，明确了DES的半致死浓度为0.3%~0.35%，0.5%为致死浓度；通过同一品种、相同DES浓度、不同处理时间试验，明确了DES最佳处理时间为10~14 h；通过相同DES浓度、相同处理时间、不同品种试验，明确了不同品种对DES的敏感程度不同，它们的半致死浓度和致死浓度都有一定差异。虽然不同基因型对DES的敏感程度不同，但是绝大多数材料适宜的DES处理浓度范围为0.3%~0.35%。

Studies on Inducement of Sorghum Seeds by Diethyl Sulfate

This paper studied the effects of different DES concentration and different soaking time on sorghum seeds of different genotypes for the purpose of detecting DES mutagenic effects the experimental results of the same genotype, the same soaking time and the different DES concentration showed that the suitable concentration of DES treating seeds was 0.3%-0.35%, 0.5% were lethal concentration. The experimental results of the same genotype, the same DES concentration and the different soaking time showed that the optimal soaking time of DES was 10-14 h. The experimental results of the same soaking time, the same DES concentration and the different genotypes showed that the sensitive degree of different genotype to DES was different and their lethal concentration and semi-lethal concentration are different. Although different genotypes have different sensitive degree to DES, but the appropriate DES concentrations for the majority of sorghum materials range from 0.3%-0.35%.