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| 家猪染色体脆性位点的初步研究 | |
| 引用本文： | 雷初朝,王德堂,辛晓燕. 家猪染色体脆性位点的初步研究[J]. 西北农林科技大学学报(自然科学版), 1998, 26(5): 5-8 |
| 作者姓名： | 雷初朝 王德堂 辛晓燕 |
| 作者单位： | 西北农业大学动物科学与动物医学学院，第四军医大学妇产科 |
| 摘 要： | 采用外周血淋巴细胞培养法及常规染色技术，以ＴｄＲ和ＦＵｄＲ作诱导剂，对家猪染色体脆性位点进行初步研究。结果显示，ＴｄＲ诱导家猪脆性位点的有效处理浓度为６０～８０ｍｇ·Ｌ－１，脆性位点的表达频率为６５％左右。ＴｄＲ型脆性位点主要发生于１ｑ，２ｑ，６ｑ，１３ｑ，１６ｑ染色体，ＦＵｄＲ型脆性位点主要集中于１ｑ，２ｑ，６ｑ，１３ｑ，１４ｑ，１５ｑ，１６ｑ染色体，家猪的脆性位点表达可能存在个体差异。并对脆性位点的类型及生物学意义进行了简要讨论。 |
| 关 键 词： | 家猪 染色体 脆性位点 |
A Primary Study on Fragile Sites in Domestic Pig Chromosome |
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| Lei Chuzhao Wang Detang Xin Xiaoyan Yang Gongshe Zhan Tiesheng. A Primary Study on Fragile Sites in Domestic Pig Chromosome[J]. Journal of Northwest A&F University（Natural Science Edition）, 1998, 26(5): 5-8 | |
| Authors: | Lei Chuzhao Wang Detang Xin Xiaoyan Yang Gongshe Zhan Tiesheng |
| Affiliation: | 1 (1 College of Animal Science and Veterinary Medicine,Northwestern Agricultural University,Yangling,Shaanxi 712100) (2 Department of Obstetrics and Gynecology,The Fourth |
| Abstract: | By using the peripheral blood lymphocyte culture method and common staining technology in this experiment,a primary study on fragile sites in domestic pig chromosome is done with Thymidine (TdR) and Fluorodeoxyuridine (FUdR) as inducer.The result shows that a concentration of 60-80 mg of TdR L -1 of medium is recommended as the optimum for fragile sites expression in domestic pig and the expression rate of fragile sites is about 65 percent.The TdR sensitive fragile sites in domestic pig are identified on Chromosomes 1q,2q,6q,13q and 16q,whereas the FUdR sensitive fragile sites are identified on Chromosomes 1q,2q,6q,13q,14q,15q and 16q.Individual differences may exist in the expression of fragile sites in domestic pigs.The classification and biological significance of fragile sites are also briefly discussed. |
| Keywords: | domestic pig chromosome fragile site |
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