| 阿司匹林与DNA相互作用的光谱研究 |
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| 引用本文： | 刘炜,毕和平,张连华,汤颖,陈光英.阿司匹林与DNA相互作用的光谱研究[J].安徽农业科学,2009,37(15):6837-6838. |
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| 作者姓名： | 刘炜 毕和平 张连华 汤颖 陈光英 |
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| 作者单位： | 海南师范大学化学与化工学院,海南省热带药用植物化学重点实验室,海南,海口,571158;西安石油大学化学化工学院,陕西,西安,710065 |
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| 基金项目： | 海南省重点科技项目(070207); 海南师范大学博士启动基金资助项目(103307); 海南省教育厅项目(Hjkj2009-39); 海南省有机化学重点学科开放基金 |
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| 摘 要： | 目的]研究阿司匹林（ASP）与DNA相互作用的机理。方法]在pH值为4．5的Tnis—HCl缓冲溶液体系中，应用荧光光谱法和紫外光谱技术研究ASP和DNA分子间的相互作用；利用Stem-Volmer方程探讨DNA对阿司匹林的荧光猝灭机制。结果]DNA与ASP之间存在较强的相互作用，25qc时结合常数为2．17×10^6L／mol，结合位点数为1．7735，结合方式是ASP通过嵌入结合的方式与DNA结合。结论]该研究可为进一步研究ASP的药理活性提供重要信息。
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| 关 键 词： | 阿司匹林 DNA 荧光猝灭 机制 |
Study on the Interaction between Aspirin and DNA by Spectrometric Method |
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| LIU Wei et al.Study on the Interaction between Aspirin and DNA by Spectrometric Method[J].Journal of Anhui Agricultural Sciences,2009,37(15):6837-6838. |
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| Authors: | LIU Wei |
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| Institution: | LIU Wei et al(Hainan Key Lab.of Tropical Medicinal Herb , Chemistry,College of Chemistry , Chemical Engineering,Hainan Normal University,Haikou,Hainan 571158) |
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| Abstract: | Objective]The research aimed to study the interaction mechanism between aspirin and DNA.Method]The interaction of aspirin and DNA was studied using UV spectrometry and fluorescence spectrometry.The quenching mechanism of fluorescence of aspirin by DNA was discussed through Stern-Volmer equation.Result]The results showed that there were strong interaction between aspirin and DNA.The binding constant was 2.17×106 L/mol at 25 ℃.The number of binding sites was 1.773 5.And aspirin binded with DNA in a mode of intercalative binding with the result of UV-VIS spectrum.Conclusion]This study could provide important information for further study about the pharmacological activity of aspirin. |
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| Keywords: | Aspirin DNA Fluorescence quenching Mechanism |
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