| 猪繁殖与呼吸综合征病毒江苏分离株ORF5及Nsp2基因部分序列分析 |
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| 引用本文： | 迟兰,崔盟,王寿利,吴艳涛,刘秀梵.猪繁殖与呼吸综合征病毒江苏分离株ORF5及Nsp2基因部分序列分析[J].中国兽医学报,2010,30(2). |
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| 作者姓名： | 迟兰 崔盟 王寿利 吴艳涛 刘秀梵 |
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| 作者单位： | 1. 扬州大学,农业部畜禽传染病学重点开放实验室,江苏扬州,225009;徐州生物工程高等职业学校,江苏,徐州,221006
2. 扬州大学,农业部畜禽传染病学重点开放实验室,江苏扬州,225009 |
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| 基金项目： | 国家“十一五”科技支撑计划资助项目(2006BAD06A12) |
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| 摘 要： | 对2006—2007年分离于江苏省部分发病猪场的PRRSV,采用RT-PCR技术,进行ORF5基因序列以及Nsp2基因部分序列的扩增、克隆并测序。应用DNAStar软件将测序结果与已发表的国内外参考毒株进行比对分析。结果表明,分离的21株病毒ORF5基因大小均为603bp;与国内分离株之间同源性为87.7%～97.0%,与国外美洲型分离株同源性为85.6%～90.5%;21株PRRSV分离株与欧洲型毒株之间的同源性仅为54.1%～56.6%。14株用于扩增Nsp2基因部分序列的PRRSV在全基因组第2778～2780位及第2947～3033位分别缺3个和87个碱基,其中的2个江苏扬州分离株在第2747～2836位又缺失了90个碱基;14个毒株之间同源性为93.2%～99.0%,与VR-2332相比同源性为74.9%～78.5%,与国内河北、河南等地2006年的PRRSV分离株相比同源性为94.1%～99.1%。
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| 关 键 词： | 猪繁殖与呼吸综合征病毒 ORF5 Nsp2 序列分析 |
Sequence analysis of PRRSV (Jiangsu strains) ORF5 and partial of Nsp2 gene |
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| CHI Lan,CUI Meng,WANG Shou-li,WU Yan-tao,LIU Xiu-fan.Sequence analysis of PRRSV (Jiangsu strains) ORF5 and partial of Nsp2 gene[J].Chinese Journal of Veterinary Science,2010,30(2). |
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| Authors: | CHI Lan CUI Meng WANG Shou-li WU Yan-tao LIU Xiu-fan |
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| Institution: | CHI Lan1,2,CUI Meng1,WANG Shou-li1,WU Yan-tao1,LIU Xiu-fan1 (1.Key Laboratory of Animal Infectious Disease of Agricultural Ministry,Yangzhou University,Yangzhou,Jiangsu 225009,China,2.Xuzhou Higher Professional School of Bioengineering,Xuzhou,Jiangsu 221006,China) |
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| Abstract: | ORF5 of 21 PRRSV isolates from Jiangsu province of China in 2006-2007 and Nsp2 gene of 14 isolates were amplified by RT-PCR.The PCR products were cloned and sequenced.The results showed that ORF5 gene of all the isolates were 603 bp in length.The 21 isolates had an identity of 98.5%-100.0%,simultaneously had an identity of 87.7%-97.0% and 85.6%-90.5% with domestic isolations and American strains,respectively.the 21 isolates has an identity of 54.1%-56.6% with European strains.According to Nsp2 genome analys... |
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| Keywords: | PRRSV ORF5 Nsp2 sequence analysis |
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