| 苹果褐腐病菌中草药抑菌剂的筛选及提取条件研究 |
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| 引用本文： | 李海燕,张显龙,关丽杰,邵双,马金姗.苹果褐腐病菌中草药抑菌剂的筛选及提取条件研究[J].安徽农业科学,2009,37(10):4538-4540. |
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| 作者姓名： | 李海燕 张显龙 关丽杰 邵双 马金姗 |
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| 作者单位： | 沈阳化工学院环境与生物工程学院,辽宁沈阳,110142 |
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| 基金项目： | 辽宁省教育厅高等学校科学研究项目(2004D205) |
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| 摘 要： | 目的]为中草药抑菌剂的开发提供参考依据。方法]采用菌丝生长速率法测定了19种中草药乙醇提取物对苹果褐腐病菌的抑制活性。结果]不同中草药提取物对苹果褐腐病菌的抑制活性存在一定差异。在中草药提取物浓度为0.1 mg/ml时,19种中草药中,马兜铃提取物对苹果褐腐病菌菌丝生长的抑制效果最好,抑制率高达49.64%;其次是鸦胆子、射干和葛根,抑制率均在20%以上;其余中草药提取物对苹果褐腐病菌均未表现出明显的抑制作用,其抑制率均在20%以下。通过单因素试验优化了马兜铃的提取条件,即以50%乙醇为溶剂、料液比为1∶5、提取温度为30℃、提取时间为4 h。结论]马兜铃体内的有效成分具有强烈的抑菌活性,具有开发潜力。
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| 关 键 词： | 中草药提取物 马兜铃 抑制活性 提取条件 |
Research on Screening Chinese Herbal Fungicides for Monilia fructigena and Their Extraction Conditions |
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| LI Hai-yan et al.Research on Screening Chinese Herbal Fungicides for Monilia fructigena and Their Extraction Conditions[J].Journal of Anhui Agricultural Sciences,2009,37(10):4538-4540. |
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| Authors: | LI Hai-yan |
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| Institution: | LI Hai-yan et al(School of Environmental , Biological Engineering,Shenyang Institute of Chemical Technology,Shenyang,Liaoning 110142) |
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| Abstract: | Objective] The purpose was to supply reference foundation for developing Chinese herbal fungicides.Method] The inhibitory activities of ethanol extracts of 19 Chinese herbs on Monilia fructigena were determined by mycelium growth rate method.Result] The inhibitory activities of different Chinese herbal extracts on M.fructigena had some difference.When the concn.of Chinese herbal extracts was 0.1 mg/ml,the inhibitory effect of Aristolochia debilis extracts on the mycelium growth of M.fructigena was best a... |
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| Keywords: | Chinese herb extracts Aristolochia debilis Inhibitory activity Extraction condition |
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