兔病毒性出血症4种灭活疫苗的研制及效果比较

目的]研制兔病毒性出血症新型疫苗.方法]分别以黄芪多糖、蜂胶、铝胶、生理盐水为佐剂,研制兔病毒性出血症黄芪多糖佐剂、蜂胶佐剂、铝胶佐剂以及常规灭活疫苗,并从安全、免疫效果等方面对这4种疫苗进行比较.结果]4种疫苗均有良好的安全性.免疫后7d4种疫苗产生的特异性抗体效价均逐步升高,攻毒保护率均达到100％.免疫后14 d黄芪多糖佐剂抗体效价显著高于蜂胶佐剂、铝胶佐剂以及常规灭活疫苗.免疫后180 d黄芪多糖佐剂疫苗的保护率达75％,蜂胶佐剂与铝胶佐剂可达到50％,常规灭活疫苗的保护率达25％.结论]这4种疫苗均具有良好的安全性和免疫原性,其中黄芪多糖灭活苗的免疫效果最好.

Development and Efficacy Comparison of Four Kinds of Inactivated Vaccines against Rabbit Hemorrhagic Disease

Objective] The research aimed to develop new vaccine against rabbit hemorrhagic disease. Method] Using Astragalus polysac- charides, propolis, alumina gel, physiological saline as adjuvants, Astragalus polysacchafide adjuvant, propolis adjuvant, alumina gel adju- vant and conventional inactivated vaccines against rabbit hemorrhagic disease were prepared, and the safety and immunity effect of these four kinds of vaccines were compared. Result] These four kinds of vaccine all had good safety. On the 7th day after immunization, the specific antibody titers produced by four kinds of vaccines were gradually increased and their immunprotective rate reached 100%. On the 14th day af- ter immunization, the antibody titer of astragalus polysaccharide adjuvant was significantly higher than that of propolis adjuvant, alumina gel adjuvant and conventional inactivated vaccine, On the 180th day after immunization, the protection rate of astragalus polysaccharide adjuvant vaccine reached 75% , and that of propolis adjuvant and alumina gel could reach 50% , while the protection rate of conventional inactivated vaccine rate was only 25%. Conclusion] These four kinds of vaccines showed good safety and immunogenicity and the immune effect of as- tragalus polysaccharide inactivated vaccine was better.