| 橡胶树胶乳几丁质酶基因表达的品种差异 |
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| 引用本文： | 黄瑾,校现周,金志强.橡胶树胶乳几丁质酶基因表达的品种差异[J].热带作物学报,2004,25(1):1-5. |
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| 作者姓名： | 黄瑾 校现周 金志强 |
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| 作者单位： | ①华南热带农业大学农学院 海南儋州571737；
②农业部热带作物栽培生理学重点实验室 海南儋州571737；
③中国热带农业科学院热带作物生物技术国家重点实验室 海南海口571101 |
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| 摘 要： | 为了探讨不同品系橡胶树胶乳中几丁质酶基因在表达水平上的差异,利用聚合酶链式反应(PCR)技术,克隆得到长为935bp的橡胶树几丁质酶基因,对其进行序列测定。结果表明,该基因序列与EvertBokma(2001)发表的橡胶树几丁质酶基因序列一致。此外,从3个不同品系橡胶树胶乳中提取总RNA,以已克隆的基因为探针进行Northern狭线杂交。结果表明:3个不同排胶特性品系PR107,GT1,RRIM600的几丁质酶基因杂交信号强度表现为PR107GT1>RRIM600。
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| 关 键 词： | 巴西橡胶树 几丁质酶 狭线杂交 乙烯利 |
| 修稿时间： | 2003年4月17日 |
Differences of Chitinase Gene Expression in Latex from 3 Hevea Clones |
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| Huang Jin, Xiao Xianzhou Jin Zhiqiang.Differences of Chitinase Gene Expression in Latex from 3 Hevea Clones[J].Chinese Journal of Tropical Crops,2004,25(1):1-5. |
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| Authors: | Huang Jin Xiao Xianzhou Jin Zhiqiang |
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| Institution: | ①College of Agronomy, SCUTA, Danzhou. Hainan 571737;
②Ministry of Agriculture Key Laboratory for Tropical Crop Physiology, CATAS, Danzhou, Hainan 571737;
③State Key Laboratory for Tropical Crops Biotechnology, Haikou 571101 |
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| Abstract: | The chitinase gene (PGCHI) of rubber trees was amplified by PCR, the nucleotide sequence of which was found to be consistent with that p that the expression level of chitinase gene was evidently different among the three clones of rubber tree investigated. The difference in the intensity of hybridization signal with PGCHI in the order from weak to strong was PR107, GT1 and RRIM600. After being treated with ethrel, the intensity of the signal changed and the order from weak to strong became RRIM600, GT1 and PR107. This change in the signal intensity well matched the change of level of chitinase activity under the same ethrel treated conditions. |
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| Keywords: | Hevea brasiliensis chitinase ethrel slot hybridization |
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