| 几种内生真菌DNA提取方法的比较 |
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| 引用本文： | 吴发红,黄东益,黄小龙,周 鑫,程文杰. 几种内生真菌DNA提取方法的比较[J]. 中国农学通报, 2009, 25(8): 62-64 |
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| 作者姓名： | 吴发红 黄东益 黄小龙 周 鑫 程文杰 |
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| 作者单位： | 海南大学农学院,海南儋州,571737 |
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| 摘 要： | 通过三种内生真菌DNA提取方法的比较，即CTAB法、SDS法、改良的CTAB法，结果表明三种方法都能得到目的DNA，而且得率都不错，但改良的CTAB法效果最好，DNA纯度高且质量也好。用改良的CTAB法所提取的DNA作为模板，进行PCR扩增，得到了清晰的单一的扩增普带，完全可用于酶切、测序等后续实验。
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| 关 键 词： | 苹果 苹果 花粉管通道 质粒 基因转化 |
| 收稿时间： | 2009-02-18 |
| 修稿时间： | 2009-03-03 |
Comparing Study on several Methods for DNA Extraction from endophytic fungi |
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| Wu Fahong,Huang Dongyi,Huang Xiaolong,Zhou Xin,Cheng Wenjie. Comparing Study on several Methods for DNA Extraction from endophytic fungi[J]. Chinese Agricultural Science Bulletin, 2009, 25(8): 62-64 |
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| Authors: | Wu Fahong Huang Dongyi Huang Xiaolong Zhou Xin Cheng Wenjie |
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| Affiliation: | (Academy of Agriculture, Hainan University, Danzhou Hainan 571737) |
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| Abstract: | In this study, three different methods were used to extract DNA from endophytic fungi: CTAB,SDS and the improved CTAB. Quality and quantity of the extracted DNA were compared for the there methods. The results showed that all of the there methods could obtain the DNA, but the improved CTAB could obtain the highest quantity of the purest fungal DNA. The purest DNA were amplified by PCR .The clear and singal gel fragment showed that the extracted DNA could usde for the other biological experiments. |
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| Keywords: | CTAB SDS |
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