| 紫外分光光度法测定大豆总异黄酮的含量 |
| |
| 引用本文： | 袁金斌,卢建中.紫外分光光度法测定大豆总异黄酮的含量[J].大豆科学,2004,23(2):147-150. |
| |
| 作者姓名： | 袁金斌 卢建中 |
| |
| 作者单位： | 1. 江西中医学院中药制药研究中心,南昌,330006
2. 江中制药集团技术部,南昌,330077 |
| |
| 摘 要： | 建立了测定大豆提取物及以大豆展品黄酮为原料的保健食品中大豆总异黄酮的紫外分光光度法.以染料木素为标准品,优选出325nm为测量波长,以染料木素计量试样中的大豆总异黄酮.大豆提取物中大豆总异黄酮的含量为38.1%,加标回收率为102.3%;某保健品中大豆总总异黄酮的含量为4.76%,加标回收率为99.8%.方法简便、准确、重现性好,适用于保健品的日常分析和质量控制.
|
| 关 键 词： | 大豆异黄酮 分光光度法 测定 |
| 文章编号： | 1000-9841(2004)02-0147-04 |
DETERMINATION OF SOYBEAN ISOFLAVONE WITH ULTRA- VIOLET SPECTROPHOTOMETRY |
| |
| Yuan Jinbin,Lu Jianzhong.DETERMINATION OF SOYBEAN ISOFLAVONE WITH ULTRA- VIOLET SPECTROPHOTOMETRY[J].Soybean Science,2004,23(2):147-150. |
| |
| Authors: | Yuan Jinbin Lu Jianzhong |
| |
| Institution: | Yuan Jinbin 1 Lu Jianzhong 2 |
| |
| Abstract: | A UV spectrphotometry used for determination of soybean isoflavone was established. Genistein was selected as the reference material, and the samples were determined at 325 nm. Influencing factors were dicussed and optimized. The total content of soybean isoflavones in soybean extracts is 38.1% with mean recovery 102.3%, and the result for the function food is 4.76% with mean recovery 99.9%.This method is suitable for the routine analysis and quality control of the function food with easy-to-handle, good accuracy and reproducibility. |
| |
| Keywords: | Soybean isoflavone UV spectrophotometry Determination |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
