| 利用分子标记检测霍山石斛不同继代次数试管苗的遗传稳定性 |
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| 引用本文： | 邱婧,樊洪泓,秦自清,林毅,蔡永萍.利用分子标记检测霍山石斛不同继代次数试管苗的遗传稳定性[J].分子植物育种,2008,6(3):532-536. |
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| 作者姓名： | 邱婧 樊洪泓 秦自清 林毅 蔡永萍 |
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| 作者单位： | 安徽农业大学生命科学学院,合肥,230036 |
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| 基金项目： | 安徽省教育厅自然科学基金 |
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| 摘 要： | 分别以霍山石斛成熟种子、茎段和愈伤组织为试管苗的初始材料进行继代培养,利用RAPD、ISSR以及DALP3种分子标记方法对不同继代次数（1-4代）的霍山石斛试管苗进行分析,结果表明：以成熟种子和茎段为材料的继代培养,其试管苗后代在所检测的范围内未探查到变异,具有较高的遗传稳定性。以愈伤组织为材料的继代培养,其试管苗后代从第4代开始,部分引物带型发生变化,但变化甚微,仅是带型强弱的改变。以愈伤组织为材料的继代培养物种内存在一定的变异,但在4代以内仍可稳定遗传。3种继代方式比较之下,以成熟种子和茎段为材料的继代培养是更能稳定遗传的组培快繁方式。
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| 关 键 词： | 霍山石斛 试管苗 继代次数 遗传变异 分子标记 |
The Study on the Genetic Stability of D. huoshanense Repeatedly Subcultured in vitro by Using Molecular Markers |
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| Qiu Jing,Fan Honghong,Qin Ziqing,Lin Yi,Cai Yongping.The Study on the Genetic Stability of D. huoshanense Repeatedly Subcultured in vitro by Using Molecular Markers[J].Molecular Plant Breeding,2008,6(3):532-536. |
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| Authors: | Qiu Jing Fan Honghong Qin Ziqing Lin Yi Cai Yongping |
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| Institution: | Qiu Jing Fan Honghong Qin Ziqing Lin Yi Cai YongpingSchool of Life Science,Anhui Agriculture University,Hefei,230036 |
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| Abstract: | The genetic stability of Dendrobium huoshanense repeatedly subcultured in vitro for one to four times with three different original materials of mature seed, stem segment, and callus, was examined by using RAPD, ISSR and DALP. The results showed that no variation was detected within the descends of D. huoshanense repeatedly subcultured in vitro with materials of mature seed and stem segment, which revealed that high genetic stability was maintained. The slight variations of some primer bands were detected s... |
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| Keywords: | Dendrobium huoshanense Seedling Times of subculturing Genetic variation Molecular marker |
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