利用淀粉凝胶电泳技术鉴定外源DNA导入后代

利用淀粉凝胶电泳技术，对外源ＤＮＡ直接导入栽培大豆的变异后代，进行了磷酸己糖异构化酶（ＧＰＩ）、异柠檬酸脱氢酶（ＩＤＨ）和葡糖磷酸变位酶（ＰＧＭ）的同工酶酶谱分析。实验结果表明：将向日葵的ＤＮＡ导入栽培大豆后，三种酶的谱带清楚，后代与受体及供体间谱带存在差异。有的出现了供体特异带，有的出现了供体和受体所不含有的新型带，有的酶谱带加强或减弱，这说明外源ＤＮＡ已导入到受体基因组中，并在不同程度和不同方式上得到了表达。

EXAMINATION OF PROGENIES OF SOYBEAN RECEIVED EXOGENOUS DNA WITH STARCH GELELECTROPHORESIS

With the method of starch Gel Electrophoresis, analysis of patterns of GPI, IDH and PGM were made in variant progenies of soybean cultivars which exogenous DNA was introduced directly. The results showed that in soybean received DNA of sunflower, patterns of three enzymes were clear, and there were differences among patterns of the progeny, receptor and donor. Some of the progenies had the special bands from donor, some had new bands that didn't belong to donor or receptor and some had stronger or weaker bands. These indicate that the fragments of exogenous DNA have inserted into the genome of the receptor and expressing.