| 蒙古黄芪的组织培养及辐射诱变研究 |
| |
| 引用本文： | 赵秀娟,张红霞.蒙古黄芪的组织培养及辐射诱变研究[J].安徽农业科学,2009,37(9):3939-3941. |
| |
| 作者姓名： | 赵秀娟 张红霞 |
| |
| 作者单位： | 内蒙古科技大学生物与化学工程学院,内蒙古包头,014010;内蒙古科技大学生物与化学工程学院,内蒙古包头,014010 |
| |
| 基金项目： | 内蒙古科技大学校内基金项目(KJ200611) |
| |
| 摘 要： | 目的]研究蒙古黄芪的组织培养技术及辐射诱变对愈伤组织诱导的影响。方法]以蒙古黄芪无菌苗的不同外植体（叶片、子叶、下胚轴）进行愈伤组织诱导,并对子叶和下胚轴进行辐射诱变研究。结果]3种外植体分别在MS＋6-BA 2.0 mg/L＋NAA 2.0 mg/L、LS＋6-BA 2.0 mg/L＋NAA 0.1 mg/L、MS＋6-BA 2.0 mg/L＋NAA 2.0 mg/L培养基上的愈伤组织诱导率较高 下胚轴和子叶的最佳诱变时间均为15 min 随辐射诱变时间的增加,下胚轴愈伤组织的长势增强,但超过一定的诱变时间愈伤组织长势减弱且诱导率降低。结论]该研究为蒙古黄芪的组织培养和诱变育种提供科学依据。
|
| 关 键 词： | 蒙古黄芪 组织培养 辐射诱变 |
Study on Tissue Culture and Radiation Mutation of Astragalus membranaceus Bge. var. mongholicus (Bge.) Hsiao |
| |
| ZHAO Xiu-juan et al.Study on Tissue Culture and Radiation Mutation of Astragalus membranaceus Bge. var. mongholicus (Bge.) Hsiao[J].Journal of Anhui Agricultural Sciences,2009,37(9):3939-3941. |
| |
| Authors: | ZHAO Xiu-juan |
| |
| Institution: | ZHAO Xiu-juan et al (Biological , Chemical Engineering School,Inner Mongolia University of Science , Technology,Baotou,Inner Mongolia 014010) |
| |
| Abstract: | Objective] The research aimed to study the tissue culture technology and callus induction by radiation mutation of Astragalus membranaceus Bge.var.mongholicus(Bge.) Hsiao.Method] With the different parts of Astragalus membranaceus Bge.var.mongholicus(Bge.) Hsiao aseptic seedling as explants(leaves,cotyledons,hypocotyls) induced callus,and cotyledon and hypocotyls taken by the method of radiation mutation were studied.Result]The results showed that the three explants had relatively high callus induced rat... |
| |
| Keywords: | Astragalus membranaceus Bge var mongholicus(Bge ) Hsiao Tissue culture Radiation mutation |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
