| 茉莉离体快繁体系的建立 |
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| 引用本文： | 孙艳妮,汤访评,房伟民,谭国华. 茉莉离体快繁体系的建立[J]. 浙江农业学报, 2009, 21(4): 0 |
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| 作者姓名： | 孙艳妮 汤访评 房伟民 谭国华 |
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| 作者单位： | 1.南京农业大学园艺学院，江苏南京210095；2 南京市华清花卉研究所，江苏南京210014 |
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| 基金项目： | 江苏省农林厅三项工程项目 |
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| 摘 要： | 以WPM为基本培养基比较了不同6-BA和IBA配比对茉莉茎段芽诱导的影响，及不同温度条件下不同6-BA和IBA配比对茉莉芽增殖的影响；并以1／2WPM为培养基，比较了不同NAA浓度处理及不同生根方法对离体芽生根的影响。结果表明：6-BA 2．0mg／L+IBA 0．1 mg／L处理诱导率最高，达96．75％，极显著高于其他处理；而35℃下6-BA1．0mg／L+IBAO．2mg／L组合对试管苗的增殖效果最好，增殖系数为5．31；最佳生根培养方式为1／2WPM+NAA 1．0 mg／L处理7 d后转入空白培养基，进行两步法生根，生根率为98．41％。从而初步建立了茉莉的离体快繁体系。
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| 关 键 词： | 茉莉 离体快繁 诱导 |
Rapid propagation in vitro of jasmine |
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| SUN Yan-ni,TANG Fang-ping,FANG Wei-min,TAN Guo-hua. Rapid propagation in vitro of jasmine[J]. Acta Agriculturae Zhejiangensis, 2009, 21(4): 0 |
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| Authors: | SUN Yan-ni TANG Fang-ping FANG Wei-min TAN Guo-hua |
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| Affiliation: | 1 College ofHorticulture，Nanjing Agricultural University，Nanjing 210095，China；2 Nanjing Huaqin Flower Research Institute，Nanjing 210014，China |
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| Abstract: | The effects of different concentrations of 6-BA and IBA on induction of bud germination，and proliferation of jasmine at diferent temperatures were studied．Moreover, effects of different concentrations of NAA and rooting culture modes on rooting were investigated．Results showed that the highest bud induction rate of 96．75％ was obtained in the WPM medium supplemented with 2．0 mg／L 6-BA and 0．1 mg／L IBA．The best proliferation with a shoot proliferation coefficient of 5．31 was observed in the WPM medium supplemented with 1．0 mg／L 6-BA and 0．2mg／L IBA at 35℃. The optimal rooting culture mode was two-step rooting method ，i．e．，pre—culturing shoots on the rooting medium of 1／2 WPM +NAA 1．0 mg／L for 7 days, then transferring pre-cultured shoots to 1／2WPM without supplement of any plant growth regulators．The rooting rate was up to 98．41％． |
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| Keywords: | jasmine(Jasminum sambac) rapid propagation in vitro bud induction |
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