疏肝健脾解毒方对乳腺癌癌前病变肝郁证大鼠模型乳腺组织血管生成的影响

目的 研究中药疏肝健脾解毒方对乳腺癌癌前病变肝郁证大鼠模型乳腺组织微血管密度（microvessel density， MVD）、血管内皮生长因子（vascular endothelial growth factor， VEGF）及乳腺组织结构的影响，探讨其防治乳腺癌的作用机制。方法 采用二甲基苯蒽灌胃及夹尾法制备乳腺癌癌前病变肝郁证大鼠模型，随机分为模型组（蒸馏水）、中药组（疏肝健脾解毒方10.35 g/kg）、三苯氧胺组（2.0 mg/kg），另取10只为空白对照组，分别相应干预4周后，制备乳腺组织石蜡切片进行病理学观察，采用免疫组织化学方法及Western-blot法检测各组大鼠乳腺组织中MVD及VEGF表达情况。结果 免疫组化结果显示，与模型组比较，中药组及三苯氧胺组乳腺组织非典型增生现象减少，乳腺组织MVD及VEGF水平降低，差异有统计学意义（P<0.05），中药组MVD及VEGF水平稍低于三苯氧胺组，差异无统计学意义（P>0.05）；Western-blot结果显示：与模型组比较，中药组及三苯氧胺组乳腺组织非典型增生现象减少，乳腺组织MVD及VEGF水平降低，差异有统计学意义（P<0.05），中药组MVD及VEGF水平与三苯氧胺组比较差异无统计学意义（P>0.05）。结论 中药疏肝健脾解毒方可在一定程度上降低乳腺癌癌前病变乳腺组织非典型增生倾向，其机制可能与降低VEGF水平、抑制新生血管生成有关。

Effects of Shugan Jianpi Jiedu Formula on Angiogenesis of Breast Tissue in Breast Cancer Rat Model with Precancerous Lesion of Liver Qi Stagnation Syndrome

Objective To study on effects of Shugan Jianpi Jiedu Formula on microvessel density (MVD), vascular endothelial growth factor (VEGF) and breast tissue structure in breast cancer rat model with precancerous lesion of liver Qi stagnation syndrome, so as to explore its mechanism in prevention and treatment of breast cancer. Methods The breast cancer rat model with precancerous lesion of liver Qi stagnation syndrome was prepared by intragastric administration of dimethyl benzanthracene and tail clamping method. The rats were randomly divided into a model group (distilled water), a Chinese materia medica group (Shugan Jianpi Jiedu Formula 10.35 g/kg), a tamoxifen group (2.0 mg/kg) and a blank control group (n=10). Paraffin sections of breast tissue were prepared for pathological observation. MVD and VEGF expressions in breast tissue of rats were detected by immunohistochemical method and Western-blot. Results Immunohistochemical results showed that compared with the model group, atypical hyperplasia of breast tissue in the Chinese materia medica group and the tamoxifen group was decreased, breast tissue MVD and VEGF levels were decreased, and the differences were statistically significant (P<0.05). The MVD and VEGF levels in the Chinese materia medica group were slightly lower than those in the tamoxifen group, and there was no statistical significance in the difference (P>0.05). Western-blot results showed that compared with the model group, atypical hyperplasia of breast tissue in the Chinese materia medica group and the tamoxifen group was decreased, breast tissue MVD and VEGF levels were decreased, and the differences were statistically significant (P<0.05). The differences of MVD and VEGF levels between the Chinese materia medica group and the tamoxifen group were not statistically significant (P>0.05). Conclusion Shugan Jianpi Jiedu Formula can decrease atypical hyperplasia tendency in breast tissue for precancerous lesions of breast cancer to a certain extent, and the mechanism may be related to the decrease of VEGF level, and the inhibition of angiogenesis.