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| 油茶叶枯病菌的鉴定及生物学特性研究 | |
| 引用本文： | 牛晓庆,陈良秋,付登强,杨伟波,李艳,覃伟权,蒋盛军. 油茶叶枯病菌的鉴定及生物学特性研究[J]. 热带作物学报, 2013, 34(2): 352-357 |
| 作者姓名： | 牛晓庆 陈良秋 付登强 杨伟波 李艳 覃伟权 蒋盛军 |
| 作者单位： | 1. 中国热带农业科学院椰子研究所 海南省热带油料作物生物学重点实验室 海南文昌 571339 2. 中国热带农业科学院热带作物品种资源研究所,海南儋州,571737 |
| 基金项目： | 中国热带农业科学院院本级基本科研业务费（1630032012043）；海南省油茶测土配方施肥技术研究与示范推广（ZDXM20120085）。 |
| 摘 要： | 从海南五指山油茶园区采集到油茶叶枯病叶,进行病原菌分离鉴定并对其生物学特性进行研究.通过对其培养特性、形态特征的观察及rDNA的转录间隔区(ITS)序列测定,与Genbank中同源性较高的菌株进行序列比对,将该病原菌OC-12确定为小孢拟盘多毛孢(Pestalotiopsis microspora).生物学特性研究结果表明:OC-12菌丝在PDA培养基上生长最佳,在crapek培养基上不产孢,在燕麦培养基上最适合产孢.菌丝生长和分生孢子产生的适宜温度分别为20～30℃和25℃,适宜pH值为4～11.供试碳源中果糖较利于菌丝生长,D-葡萄糖较利于孢子产生；供试氮源中蛋白胨和硝酸钾较利于菌丝生长,且蛋白胨较利于孢子产生.分生孢子致死温度为52℃,10 min. |
| 关 键 词： | 油茶 小孢拟盘多毛孢菌 鉴定 生物学特性 |
Identification and Characterization of Camellia oleifera Leaf Blight Disease Pathogen |
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| NIU Xiaoqing,CHEN Liangqiu,FU Dengqiang,YANG Weibo,LI Yan,QIN Weiquan and JIANG Shengjun. Identification and Characterization of Camellia oleifera Leaf Blight Disease Pathogen[J]. Chinese Journal of Tropical Crops, 2013, 34(2): 352-357 | |
| Authors: | NIU Xiaoqing CHEN Liangqiu FU Dengqiang YANG Weibo LI Yan QIN Weiquan JIANG Shengjun |
| Affiliation: | Coconut Research Institute, Chinese Academy of Tropical Agricultural Sciences / Hainan Key Laboratory for Biology of Tropical Oil Crops;Coconut Research Institute, Chinese Academy of Tropical Agricultural Sciences / Hainan Key Laboratory for Biology of Tropical Oil Crops;Coconut Research Institute, Chinese Academy of Tropical Agricultural Sciences / Hainan Key Laboratory for Biology of Tropical Oil Crops;Coconut Research Institute, Chinese Academy of Tropical Agricultural Sciences / Hainan Key Laboratory for Biology of Tropical Oil Crops;Coconut Research Institute, Chinese Academy of Tropical Agricultural Sciences / Hainan Key Laboratory for Biology of Tropical Oil Crops;Coconut Research Institute, Chinese Academy of Tropical Agricultural Sciences / Hainan Key Laboratory for Biology of Tropical Oil Crops;ropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences |
| Abstract: | |
| Keywords: | Camellia oleifera Pestalotiopsis microspora Identification Biological characteristic |
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