本源微生物修复稠油污染土壤最佳生态条件优选试验

目的]探讨本源微生物修复稠油污染土壤最佳生态条件。方法]从辽河油田稠油污染土壤中分离出本源微生物高效降解菌株,首先通过对pH、稠油浓度、营养元素、接种量等因素进行单因素试验,然后根据单因素试验结果,进一步进行正交试验得出本源微生物降解稠油污染土壤的最佳生态条件。结果]影响微生物降解稠油污染土壤降解率因素的主次顺序依次为石油浓度接种量pHKNO3KH2PO4;微生物的最佳生态降解条件为:KNO3为0.45 g,K2HPO4为1.10 g,pH=8,接种量为2 ml,石油浓度为0.2%,此时降解率高达61.87%。结论]该研究为稠油污染土壤的微生物修复提供了理论依据。

Optimization of Ecological Conditions for Heavy Oil-contaminated Soil Remediation by an Indigenous Microorganism

Objective] The study aimed to discuss the optimal ecological conditions for heavy oil-contaminated soil remediation by an indige-nous microorganism. Method ] Indigenous bacteria segregated from heavy oil-contaminated soil in Liaohe River basin were used to restore the heavy oil-contaminated soil. Firstly, the effecls of pH, oil cuneentration, nutrient elements and inoeulum volume on degradation efficiency of petroleum hydrocarbons in the soil were analyzed through a single factor test, and the optimal ecological conditions for heavy oil-cuntuminated soil remediation were determined through an orthogonal test. Result ] Oil concentration was the most important factor influencing the degrada-tion efficiency of petroleum hydn）earhons, followed by inoeulum volume and pH. The optimal ecological conditions for heavy oil-contaminated soil remediation were as follows： dosages of KNO4 and KH2 PO4 were 0.45 and 0. 10 g respectively; pH was 8 ; inoculum wdume was 2 ml; oil concentration was 0. 2%. Under the optimal ecological conditions, the degradation efficiency reaehed 61.87%. Cunelusion] The research eouht provide scientific references for microbial remediation of heavy oil-cootaminated soil.