| 吉氏巴贝斯虫mRNA的磁性分离及cDNA合成 |
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| 引用本文： | 陈启军,李德昌,阎仲堂.吉氏巴贝斯虫mRNA的磁性分离及cDNA合成[J].中国兽医学报,1993(3). |
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| 作者姓名： | 陈启军 李德昌 阎仲堂 |
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| 作者单位： | 兽医大学寄生虫病教研室
(陈启军，李德昌)，兽医大学寄生虫病教研室(阎仲堂) |
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| 摘 要： | 从感染吉氏巴贝斯虫的摘脾犬的红细胞内提纯虫体,用异硫氰酸胍法提取虫体总RNA,再以Poly(A)~ mRNA磁性分离系统提取mRNA.最后以NotⅠPrimer-Adaptor为引物反转录出cDNA片段.琼脂糖凝胶电泳显示,所合成的cDNA分子量范围由100到6500bP,且绝大部分大于500bP.
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| 关 键 词： | 吉氏巴贝斯虫 总RNA mRNA cDNA 磁性分离 |
mRNA Magnetic Isolation and cDNA Synthesis of Babesia gibsoni |
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| Chen Qijun et al.mRNA Magnetic Isolation and cDNA Synthesis of Babesia gibsoni[J].Chinese Journal of Veterinary Science,1993(3). |
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| Authors: | Chen Qijun |
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| Institution: | Chen Qijun et al. Department of Parasitic Disease,Veterinary College of PLA |
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| Abstract: | Babesia gibsoni was purified from infected erythrocytes of splenectomizeddog. Total RNA and mRNA were isolated from the parasites with Promega Total RNAIsolation System and PolyATract mRNA Isolation System. Finally, cDNA was reverse-synthesized with Not I Primer-Adaptor. Agarose electrophoresis showed that the dou-ble-strand cDNA fragments ranged from 100 bp to 6500 bp, most of them were longerthan 500 bp. |
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| Keywords: | Babesia gibsoni total RNA mRNA cDNA magnetic isolation |
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