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| 红参胡萝卜杂交一代及双亲的酯酶同工酶比较分析 | |
| 引用本文： | 张惠梅,胡喜来,孙治强,董斌,刘利花,李鑫.红参胡萝卜杂交一代及双亲的酯酶同工酶比较分析[J].河南农业大学学报,2006,40(4):355-358. |
| 作者姓名： | 张惠梅 胡喜来 孙治强 董斌 刘利花 李鑫 |
| 作者单位： | 1. 河南农业大学林学园艺学院,河南,郑州,450002 2. 郑州绿金园农业科技有限公司,河南,郑州,450002 3. 商丘市蔬菜办公室,河南,商丘,476000 |
| 摘 要： | 以红参胡萝卜杂交一代及其双亲为材料,采用聚丙烯酰胺凝胶不连续电泳技术,在生殖生长期分别对其吸收根、叶片、花蕾,进行了酯酶同工酶(EST)分析.结果表明,红参胡萝卜及双亲酯酶同工酶随着种株生长时间的推移而发生变化.不同阶段、不同器官的EST酶带按Rf值大小排列,共出现15条酶带,其中有4条保守酶带.红参胡萝卜酶带表现为双亲互补型,有1条在不同时期不同器官均有表达的杂种酶带,酶带清晰,与双亲不同. |
| 关 键 词： | 红参胡萝卜 酯酶同工酶 电泳 |
| 文章编号： | 1000-2340（2006）04-0355-04 |
| 收稿时间： | 2006-02-28 |
| 修稿时间： | 2006年2月28日 |
Comparative Analysis on Esterase Isozymes of Daucus carota L. "Hongshen" and Its Parents |
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| ZHANG Hui-mei,HU Xi-lai,SUN Zhi-qiang,DONG Bin,LIU Li-hua,LI Xin.Comparative Analysis on Esterase Isozymes of Daucus carota L. "Hongshen" and Its Parents[J].Journal of Henan Agricultural University,2006,40(4):355-358. | |
| Authors: | ZHANG Hui-mei HU Xi-lai SUN Zhi-qiang DONG Bin LIU Li-hua LI Xin |
| Institution: | 1. College of Forestry and Horticulture, Henan Agricuhural University, Zhengzhou 450002, China ; 2. Zhengzhou Ltijinyuan Inc.of Agricultural Technology ,Zhengzhou 450002,China; 3. Shangqiu Vegetable Office,Shangqiu 476000,China |
| Abstract: | Esterase isozymes of roots,leaves and flower buds at reproductive stage were analyzed in Daucus carota L."Hongshen" and its parents by using the discontinuous system of ployacrylamide gel electrophoresis.The results indicated that Daucus carota L."Hongshen".and its parents had 15 EST bands in detected tissues at developmental stages which changed with the developmental stage,four of which were constant.Daucus carota L."Hongshen"appeared parental complementary in EST bands and one of them expressed EST bands at different developmental stage in different tissues which was distinctive from its parents. |
| Keywords: | Daucus carota L Hongshen esterase isozymes electrophoresis |
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