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矮牵牛的组织培养和快速繁殖
引用本文:彭海凤,胡孔峰,方保停,张进. 矮牵牛的组织培养和快速繁殖[J]. 信阳农业高等专科学校学报, 2001, 11(1): 19-19,21
作者姓名:彭海凤  胡孔峰  方保停  张进
作者单位:信阳农业高等专科学校,河南,信阳,464000
摘    要:以矮牵牛的叶片、茎段为外植体进行组织培养。结果表明 :叶和茎段可诱导形成愈伤组织。经试验筛选出各培养阶段最适宜的培养基为 :(1)愈伤组织诱导 ,1/ 2ms + 6 -BA 1mg/L +IBA0 .0 2mg/L +琼脂 8g/L  (2 )增殖培养 ,ms+ 6 -BA 1mg/L +IBA 0 .2mg/L +琼脂 8g/L  (3)生根培养 ,1/ 2ms+IBA 0 .5mg/L +琼脂 8g/L。

关 键 词:矮牵牛  组织培养  快速繁殖
文章编号:1008-4916(2001)01-0019-01

Tissue culture and rapid propagation of common petunia
PENG Hai-feng,HU kong-feng,FANG Bao-ting,ZHANG Jin. Tissue culture and rapid propagation of common petunia[J]. Journal of Xinyang Agricultural College, 2001, 11(1): 19-19,21
Authors:PENG Hai-feng  HU kong-feng  FANG Bao-ting  ZHANG Jin
Abstract:The leaves and stem segments of common petunia as the explants were cultured.The results indicated that the leaves and stem segments could induce callus.The most appropricate media in culture stages were:(1)1/2ms 6-BA 1mg/L IBA 0.02mg/L for the formation of callus;(2)ms 6-BA 1mg/L IBA 0.2mg/L for the differentiation of buds (3)1/2ms IBA 0.5mg/L for rooting.
Keywords:common petunia  tissue culture  rapid propagation
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