亚洲百合与东方百合远缘杂交亲和性

为得到花色丰富、带有芳香气味、抗性强的OA或AO(O为东方百合杂种系,A为亚洲百合杂种系)型优势杂种,采用常规杂交和切割柱头的方法进行杂交,结果 AO型的22个组合,仅有亚洲百合‘Pollyanna’×东方百合‘Sambuca’这一组合得到了4个膨大的蒴果和40粒有胚种子,结实率为0.27%;85个LA(LA为Longiflorum hybrids×Asiatic hybrids的杂交后代)×O型组合均未得到完全膨大的果实和有胚种子;而21个OA型组合,有7个组合得到了膨大的蒴果及有胚种子。采用切割柱头的方法也未提高结实率,说明其没能有效克服其远缘杂交的障碍。通过花粉管荧光显微观察发现,亚洲百合和东方百合花粉几乎均能在母本的柱头上萌发,并沿着花柱道伸长生长,最终达到子房进入胚珠,说明AO型或OA型远缘杂交的受精障碍主要为受精后障碍。

Compatibility for Lily Distant Hybridization between Asiatic Hybrids and Oriental Hybrids

An experiment was conducted to take hybridization to get advantaged AO or OA hybrids ( A:Asiatic hybrids;O:Ori-ental hybrids ) by normal pollination technique and cut-style pollination technique .In the experiment , with Asiatic hybrids as female parent , only one combination ‘Pollyanna ’ בSambuca ’ among 22 combinations obtained fruits and embryos . While the number of swelling capsule and the rate of embryos reached 4 and 0.27%, respectively.With LA(LA:Longiflo-rum hybrids×Asiatic hybrids) hybrids as female parent , there was no fruit.However, with Oriental hybrids as female par-ent, 7 combinations obtained fruits and embryos .The method of cut-style pollination technique could not improve the seed rate, and failed to effectively overcome obstacles distant hybridization .The pollen germination and pollen tube growth were observed after pollination by fluorescence .The pollens of both Asiatic hybrids and Oriental hybrids could germinate on the stigma, and finally get into the ovule .Therefore, there was the hybridization barriers mainly after fertilization .