多年生黑麦草花粉活力和柱头可授性

以多年生黑麦草(Lolium perenne)品种‘Pinnacle’为材料,用TTC染色法和花粉管体外萌发法测定花粉在4种贮藏条件下(4℃常态、4℃干燥、-20℃常态和-20℃干燥)的活力变化;采用联苯胺—过氧化氢法测定开花前和开花后不同时段的柱头可授性。结果表明:多年生黑麦草花粉不耐贮藏,相比较而言,4℃干燥有利于其保存,7 d后花粉活力可维持在5%左右;而柱头可授性在开花前1 d至开花后5 h较强,该时间内授粉有利于受精作用,可提高结实率。

Pollen Viab ility and Stigma Receptivity of Lolium perenne

With Lolium peren e commercial variety ‘Pinnacle ’, we used TTC staining method and pollen tube germination method in vitro to measure pollen viability under four storage conditions of 4℃and room humidity, 4℃and dry condition,-20℃and room humidity , and -20℃and dry condition .And we used benzidine-hydrogen peroxide method to study stigma .The pollen of Lolium perenne was not resistant to storage , and 4℃with dry condition was beneficial to its conserve relatively . Pollen viability maintained about 5% after 7d .Stigma receptivity was strong between 1 d before blooming and 5 h after blooming, and this period was beneficial to fertilization and enhancing fruiting rate of flowers .